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DLD Polyclonal Antibody (A13296)

Publication (1) Datasheet

ABclonal:Western blot - DLD Polyclonal Antibody (A13296)
ABclonal:Immunohistochemistry - DLD Polyclonal Antibody (A13296)
ABclonal:Immunohistochemistry - DLD Polyclonal Antibody (A13296)
ABclonal:Immunofluorescence - DLD Polyclonal Antibody (A13296)

Overview

Product nameDLD Polyclonal Antibody
Catalog No.A13296
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 36-300 of human DLD (NP_000099.2).
SequenceADQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGK
Gene ID1738
Swiss protP09622
SynonymsDLD; DLDD; DLDH; E3; GCSL; LAD; PHE3; dihydrolipoamide dehydrogenase
Calculated MW43kDa/49kDa/54kDa
Observed MW54kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, BT-474, K-562, 293T, Mouse liver
Cellular locationMitochondrion matrix
Customer validation

WB(Human)

ABclonal:Western blot - DLD Polyclonal Antibody (A13296) }

Western blot - DLD Polyclonal Antibody (A13296)

Western blot analysis of extracts of various cell lines, using DLD antibody (A13296) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - DLD Polyclonal Antibody (A13296) }

Immunohistochemistry - DLD Polyclonal Antibody (A13296)

Immunohistochemistry of paraffin-embedded human liver damage using DLD antibody (A13296) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - DLD Polyclonal Antibody (A13296) }

Immunohistochemistry - DLD Polyclonal Antibody (A13296)

Immunohistochemistry of paraffin-embedded human esophageal cancer using DLD antibody (A13296) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - DLD Polyclonal Antibody (A13296) }

Immunofluorescence - DLD Polyclonal Antibody (A13296)

Immunofluorescence analysis of HeLa cells using DLD antibody (A13296). Blue: DAPI for nuclear staining.

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