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DDIT3 / CHOP Polyclonal Antibody (A11346)

Publications (2) Datasheet

ABclonal:Western blot - DDIT3 / CHOP Polyclonal Antibody (A11346)
ABclonal:Western blot - DDIT3 / CHOP Polyclonal Antibody (A11346)
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)

Overview

Product nameDDIT3 / CHOP Polyclonal Antibody
Catalog No.A11346
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-169 of human DDIT3 / CHOP (NP_004074.2).
SequenceMAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQGRTRKRKQSGHSPARAGKQRMKEKEQENERKVAQLAEENERLKQEIERLTREVEATRRALIDRMVNLHQA
Gene ID1649
Swiss protP35638
SynonymsDDIT3; CEBPZ; CHOP; CHOP-10; CHOP10; GADD153; C/EBPzeta; DDIT3 / CHOP
Calculated MW19kDa/21kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesC2C12, C6, BT-474, SKOV3, A-375, HeLa
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mouse, Rat)

ABclonal:Western blot - DDIT3 / CHOP Polyclonal Antibody (A11346) }

Western blot - DDIT3 / CHOP Polyclonal Antibody (A11346)

Western blot analysis of extracts of various cell lines, using DDIT3 antibody (A11346) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - DDIT3 / CHOP Polyclonal Antibody (A11346) }

Western blot - DDIT3 / CHOP Polyclonal Antibody (A11346)

Western blot analysis of extracts of various cell lines, using DDIT3 / CHOP antibody (A11346) at 1:1000 dilution. Both C2C12 cells and C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346) }

Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)

Immunohistochemistry of paraffin-embedded rat pancreatic islets using DDIT3 / CHOP antibody (A11346) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346) }

Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)

Immunohistochemistry of paraffin-embedded mouse testis using DDIT3 / CHOP antibody (A11346) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346) }

Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)

Immunohistochemistry of paraffin-embedded mouse pancreatic islets using DDIT3 / CHOP antibody (A11346) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346) }

Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)

Immunohistochemistry of paraffin-embedded rat testis using DDIT3 / CHOP antibody (A11346) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346) }

Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)

Immunohistochemistry of paraffin-embedded rat testis using DDIT3 / CHOP antibody (A11346) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346) }

Immunohistochemistry - DDIT3 / CHOP Polyclonal Antibody (A11346)

Immunohistochemistry of paraffin-embedded human thyroid cancer using DDIT3 / CHOP antibody (A11346) at dilution of 1:100 (40x lens).

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