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DDIT3 / CHOP Polyclonal Antibody (A0854)

Review (1) Datasheet

ABclonal:Western blot - DDIT3 / CHOP Polyclonal Antibody (A0854)
Review
ABclonal: review for DDIT3 / CHOP Polyclonal Antibody(A0854)

Overview

Product nameDDIT3 / CHOP Polyclonal Antibody
Catalog No.A0854
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human DDIT3 / CHOP (NP_004074.2).
SequenceMAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQG
Gene ID1649
Swiss protP35638
SynonymsDDIT3; CEBPZ; CHOP; CHOP-10; CHOP10; GADD153; C/EBPzeta; DNA damage inducible transcript 3
Calculated MW19kDa/21kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse testis, Mouse brain
Cellular locationCytoplasm, Nucleus
Customer validation

ABclonal:Western blot - DDIT3 / CHOP Polyclonal Antibody (A0854) }

Western blot - DDIT3 / CHOP Polyclonal Antibody (A0854)

Western blot analysis of extracts of various cell lines, using DDIT3 / CHOP antibody (A0854) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 20s.

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