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Cyclin D1 Rabbit pAb (A11022)

Publications (4) Datasheet

ABclonal:Western blot - Cyclin D1 Polyclonal Antibody (A11022)
ABclonal:Immunofluorescence - Cyclin D1 Polyclonal Antibody (A11022)
ABclonal:Immunoprecipitation - Cyclin D1 Polyclonal Antibody (A11022)

Overview

Product nameCyclin D1 Rabbit pAb
Catalog No.A11022
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human Cyclin D1 (NP_444284.1).
SequencePSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
Gene ID595
Swiss protP24385
SynonymsBCL1; D11S287E; PRAD1; U21B31; CCND1; Cyclin D1; cyclin D1
Calculated MW33kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesU-87MG, HepG2, SW480, HT-1080, Mouse kidney
Cellular locationCytoplasm, Membrane, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens)

ABclonal:Western blot - Cyclin D1 Polyclonal Antibody (A11022) }

Western blot - Cyclin D1 Polyclonal Antibody (A11022)

Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (A11022) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Cyclin D1 Polyclonal Antibody (A11022) }

Immunofluorescence - Cyclin D1 Polyclonal Antibody (A11022)

Immunofluorescence analysis of HeLa cells using Cyclin D1 antibody (A11022) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Cyclin D1 Polyclonal Antibody (A11022) }

Immunoprecipitation - Cyclin D1 Polyclonal Antibody (A11022)

Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug Cyclin D1 antibody (A11022). Western blot was performed from the immunoprecipitate using Cyclin D1 antibody (A11022) at a dilition of 1:1000.

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