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Cox2 Polyclonal Antibody (A1253)

Reviews (3)Publications (5) Datasheet

ABclonal:Western blot - Cox2 Polyclonal Antibody (A1253)
ABclonal:Immunohistochemistry - Cox2 Polyclonal Antibody (A1253)
ABclonal:Immunohistochemistry - Cox2 Polyclonal Antibody (A1253)
ABclonal:Immunofluorescence - Cox2 Polyclonal Antibody (A1253)
Review
ABclonal: review for Cox2 Polyclonal Antibody(A1253)
Review
ABclonal: review for Cox2 Polyclonal Antibody(A1253)
Review
ABclonal: review for Cox2 Polyclonal Antibody(A1253)

Overview

Product nameCox2 Polyclonal Antibody
Catalog No.A1253
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 375-604 of human Cox2 (NP_000954.1).
SequencePLLPDTFQIHDQKYNYQQFIYNNSILLEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQASIDQSRQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERSTEL
Gene ID5743
Swiss protP35354
SynonymsPTGS2; COX-2; COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2; prostaglandin G/H synthase 2
Calculated MW68kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:1000 - 1:4000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesA-549, HeLa, Mouse spleen, Mouse lung, Rat lung, Rat kidney
Cellular locationEndoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Customer validation

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IHC(unknown)

WB(Rat, Mouse)

IHC(Mouse)

ABclonal:Western blot - Cox2 Polyclonal Antibody (A1253) }

Western blot - Cox2 Polyclonal Antibody (A1253)

Western blot analysis of extracts of various cell lines, using Cox2 antibody (A1253) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - Cox2 Polyclonal Antibody (A1253) }

Immunohistochemistry - Cox2 Polyclonal Antibody (A1253)

Immunohistochemistry of paraffin-embedded human liver cancer using Cox2 antibody (A1253) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Cox2 Polyclonal Antibody (A1253) }

Immunohistochemistry - Cox2 Polyclonal Antibody (A1253)

Immunohistochemistry of paraffin-embedded mouse spleen using Cox2 antibody (A1253) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - Cox2 Polyclonal Antibody (A1253) }

Immunofluorescence - Cox2 Polyclonal Antibody (A1253)

Immunofluorescence analysis of HeLa cells using Cox2 antibody (A1253) at dilution of 1:100. Blue: DAPI for nuclear staining.

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