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CYP2E1 Rabbit pAb (A2160)

Review (1)Publication (1) Datasheet

ABclonal:Western blot - CYP2E1 Polyclonal Antibody (A2160)

Western blot analysis of extracts of various cell lines, using CYP2E1 antibody (A2160) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - CYP2E1 Polyclonal Antibody (A2160)

Immunohistochemistry of paraffin-embedded human liver cancer using CYP2E1 antibody (A2160) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CYP2E1 Polyclonal Antibody (A2160)

Immunohistochemistry of paraffin-embedded human stomach using CYP2E1 antibody (A2160) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - CYP2E1 Polyclonal Antibody (A2160)

Immunofluorescence analysis of HeLa cells using CYP2E1 antibody (A2160). Blue: DAPI for nuclear staining.

Review
ABclonal: review for CYP2E1 Rabbit pAb(A2160)

Overview

Product nameCYP2E1 Rabbit pAb
Catalog No.A2160
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1).
SequenceKVIKNVAEVKEYVSERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYTMDGITVTVADLFFAGTETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRIPAIKDRQEMPYMDAVVHEIQRFITLVPSNLPHEATRDTIFRGYLIPKGTVVVPTLDSVLYDNQEFPDPEKFKPEHFLNENGKFKYSDYFKPFSTGKRVCAGEGLARMELFLLLCAILQHFNLKPLVDPKDIDLSPIHIGFGCIPPRYKLCVIPRS
Gene ID1571
Swiss protP05181
SynonymsCYP2E1; CPE1; CYP2E; P450-J; P450C2E
Calculated MW56kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver, Mouse kidney, Rat liver
Cellular locationEndoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Customer validation

(Pheochromocytoma in mice)

WB(Mus musculus)

ABclonal:Western blot - CYP2E1 Polyclonal Antibody (A2160) }

Western blot - CYP2E1 Polyclonal Antibody (A2160)

Western blot analysis of extracts of various cell lines, using CYP2E1 antibody (A2160) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - CYP2E1 Polyclonal Antibody (A2160) }

Immunohistochemistry - CYP2E1 Polyclonal Antibody (A2160)

Immunohistochemistry of paraffin-embedded human liver cancer using CYP2E1 antibody (A2160) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CYP2E1 Polyclonal Antibody (A2160) }

Immunohistochemistry - CYP2E1 Polyclonal Antibody (A2160)

Immunohistochemistry of paraffin-embedded human stomach using CYP2E1 antibody (A2160) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CYP2E1 Polyclonal Antibody (A2160) }

Immunofluorescence - CYP2E1 Polyclonal Antibody (A2160)

Immunofluorescence analysis of HeLa cells using CYP2E1 antibody (A2160). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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