Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CYP11A1 Rabbit pAb (A16363)

Publications (4) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CYP11A1 Rabbit pAb (A16363)

Western blot analysis of extracts of Mouse testis, using CYP11A1 antibody (A16363) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - CYP11A1 Rabbit pAb (A16363)

Western blot analysis of extracts of Rat testis, using CYP11A1 antibody (A16363) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 18s.

ABclonal:Immunohistochemistry - CYP11A1 Rabbit pAb (A16363)

Immunohistochemistry analysis of paraffin-embedded rat ovary using CYP11A1 Rabbit pAb (A16363) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - CYP11A1 Rabbit pAb (A16363)

Immunofluorescence analysis of human placenta using CYP11A1 Rabbit pAb (A16363) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name CYP11A1 Rabbit pAb
Catalog No. A16363
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 40-320 of human CYP11A1 (NP_000772.2).
Sequence ISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAAWKKDRVALNQEVMAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRGILYRLLGDSKMSFEDIKANV
Gene ID 1583
Swiss prot P05108
Synonyms CYP11A; CYPXIA1; P450SCC; CYP11A1
Calculated MW 60kDa
Observed MW 50kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HEL, HeLa, Mouse testis, Rat testis
Cellular location Mitochondrion membrane
Customer validation

WB (Capra hircus, Rattus norvegicus)

IHC (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CYP11A1 Rabbit pAb images

ABclonal:Western blot - CYP11A1 Rabbit pAb (A16363)}

Western blot - CYP11A1 Rabbit pAb (A16363)

Western blot analysis of extracts of Mouse testis, using CYP11A1 antibody (A16363) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - CYP11A1 Rabbit pAb (A16363)}

Western blot - CYP11A1 Rabbit pAb (A16363)

Western blot analysis of extracts of Rat testis, using CYP11A1 antibody (A16363) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 18s.
ABclonal:Immunohistochemistry - CYP11A1 Rabbit pAb (A16363)}

Immunohistochemistry - CYP11A1 Rabbit pAb (A16363)

Immunohistochemistry analysis of paraffin-embedded rat ovary using CYP11A1 Rabbit pAb (A16363) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - CYP11A1 Rabbit pAb (A16363)}

Immunofluorescence - CYP11A1 Rabbit pAb (A16363)

Immunofluorescence analysis of human placenta using CYP11A1 Rabbit pAb (A16363) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on CYP11A1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CYP11A1. (Distance between topics and target gene indicate popularity.) CYP11A1

* Data provided by citexs.com, for reference only.

Publishing research using A16363? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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