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CYLD Polyclonal Antibody (A3821)

Datasheet

ABclonal:Western blot - CYLD Polyclonal Antibody (A3821)

Overview

Product nameCYLD Polyclonal Antibody
Catalog No.A3821
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 654-953 of human CYLD (NP_001035877.1).
SequenceTKIMKLRKILEKVEAASGFTSEEKDPEEFLNILFHHILRVEPLLKIRSAGQKVQDCYFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFPSLELNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCNTQVHLHPKRLNHKYNPVSLPKDLPDWDWRHGCIPCQNMELFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVGEYLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK
Gene ID1540
Swiss protQ9NQC7
SynonymsCYLD; BRSS; CDMT; CYLD1; CYLDI; EAC; MFT; MFT1; SBS; TEM; USPL2; CYLD lysine 63 deubiquitinase
Calculated MW107kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-87MG, HL-60, HT-1080, Mouse kidney, Mouse brain
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, perinuclear region, spindle
ABclonal:Western blot - CYLD Polyclonal Antibody (A3821) }

Western blot - CYLD Polyclonal Antibody (A3821)

Western blot analysis of extracts of various cell lines, using CYLD antibody (A3821) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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