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CXCR4 Polyclonal Antibody (A12534)

Datasheet

ABclonal:Western blot - CXCR4 Polyclonal Antibody (A12534)
ABclonal:Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534)
ABclonal:Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534)
ABclonal:Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534)
ABclonal:Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534)

Overview

Product nameCXCR4 Polyclonal Antibody
Catalog No.A12534
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-42 of human CXCR4 (NP_001008540.1).
SequenceMSIPLPLLQIYTSDNYTEEMGSGDYDSMKEPCFREENANFNK
Gene ID7852
Swiss protP61073
SynonymsCXCR4; CD184; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; WHIMS; C-X-C chemokine receptor type 4
Calculated MW39kDa/40kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesTHP-1
Cellular locationCell junction, Cell membrane, Early endosome, Late endosome, Lysosome, Multi-pass membrane protein
Customer validation

WB(Mouse)

ABclonal:Western blot - CXCR4 Polyclonal Antibody (A12534) }

Western blot - CXCR4 Polyclonal Antibody (A12534)

Western blot analysis of extracts of THP-1 cells, using CXCR4 Antibody (A12534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534) }

Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534)

Immunohistochemistry of paraffin-embedded human prostate using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534) }

Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534)

Immunohistochemistry of paraffin-embedded rat lung using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534) }

Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534)

Immunohistochemistry of paraffin-embedded rat brain using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534) }

Immunohistochemistry - CXCR4 Polyclonal Antibody (A12534)

Immunohistochemistry of paraffin-embedded mouse stomach using CXCR4 antibody (A12534) at dilution of 1:100 (40x lens).

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