Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CXCR1 Rabbit pAb (A16386)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CXCR1 Rabbit pAb (A16386)

Western blot analysis of extracts of various cell lines, using CXCR1 antibody (A16386) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CXCR1 Rabbit pAb (A16386)

Immunohistochemistry analysis of paraffin-embedded rat lung using CXCR1 antibody (A16386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CXCR1 Rabbit pAb (A16386)

Immunohistochemistry analysis of paraffin-embedded human colon using CXCR1 antibody (A16386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CXCR1 Rabbit pAb (A16386)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using CXCR1 antibody (A16386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Overview

Product name CXCR1 Rabbit pAb
Catalog No. A16386
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 250-350 of human CXCR1 (NP_000625.1).
Sequence LVSKEFLARHRVTSY
Gene ID 3577
Swiss prot P25024
Synonyms C-C; CD128; CD181; CKR-1; IL8R1; IL8RA; CMKAR1; IL8RBA; CDw128a; C-C-CKR-1; CXCR1
Calculated MW 40kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Jurkat, Raji
Cellular location Cell membrane, Multi-pass membrane protein
Customer validation

WB (Homo sapiens)

IHC (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

CXCR1 Rabbit pAb images

ABclonal:Western blot - CXCR1 Rabbit pAb (A16386)}

Western blot - CXCR1 Rabbit pAb (A16386)

Western blot analysis of extracts of various cell lines, using CXCR1 antibody (A16386) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CXCR1 Rabbit pAb (A16386)}

Immunohistochemistry - CXCR1 Rabbit pAb (A16386)

Immunohistochemistry analysis of paraffin-embedded rat lung using CXCR1 antibody (A16386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CXCR1 Rabbit pAb (A16386)}

Immunohistochemistry - CXCR1 Rabbit pAb (A16386)

Immunohistochemistry analysis of paraffin-embedded human colon using CXCR1 antibody (A16386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CXCR1 Rabbit pAb (A16386)}

Immunohistochemistry - CXCR1 Rabbit pAb (A16386)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using CXCR1 antibody (A16386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on CXCR1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CXCR1. (Distance between topics and target gene indicate popularity.) CXCR1

* Data provided by citexs.com, for reference only.

Publishing research using A16386? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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