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Cullin 1 Rabbit pAb (A2136)

Review (1)Publications (2) Datasheet

ABclonal:Western blot - Cullin 1 Rabbit pAb (A2136)

Western blot analysis of extracts of various cell lines, using Cullin 1 antibody (A2136) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Cullin 1 Rabbit pAb (A2136)

Immunofluorescence analysis of HeLa cells using Cullin 1 antibody (A2136). Blue: DAPI for nuclear staining.

Review
ABclonal: review for Cullin 1 Rabbit pAb(A2136)

Overview

Product nameCullin 1 Rabbit pAb
Catalog No.A2136
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1–3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 500-776 of human Cullin 1 (NP_003583.2).
SequenceYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA
Gene ID8454
Swiss protQ13616
SynonymsCullin 1; CUL1; cullin-1; MGC149834; MGC149835
Calculated MW89kDa
Observed MW80KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesA-549, MCF7, NIH/3T3, K-562, C6, Mouse liver, Mouse brain, Rat testis
Cellular location
Customer validation

(Human uterine stromal cells)

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - Cullin 1 Rabbit pAb (A2136) }

Western blot - Cullin 1 Rabbit pAb (A2136)

Western blot analysis of extracts of various cell lines, using Cullin 1 antibody (A2136) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - Cullin 1 Rabbit pAb (A2136) }

Immunofluorescence - Cullin 1 Rabbit pAb (A2136)

Immunofluorescence analysis of HeLa cells using Cullin 1 antibody (A2136). Blue: DAPI for nuclear staining.

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