Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Cystathionase/CTH Rabbit pAb (A6121)

Publications (4) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Cystathionase/CTH Rabbit pAb (A6121)

Western blot analysis of various lysates, using Cystathionase/CTH Rabbit pAb (A6121) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Cystathionase/CTH Rabbit pAb (A6121)

Immunofluorescence analysis of HepG2 cells using Cystathionase/CTH Rabbit pAb (A6121) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Cystathionase/CTH Rabbit pAb (A6121)

Immunoprecipitation analysis of 300 μg extracts of K-562 cells using 3 μg Cystathionase/CTH antibody (A6121). Western blot was performed from the immunoprecipitate using Cystathionase/CTH antibody (A6121) at a dilution of 1:500.

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Overview

Product name Cystathionase/CTH Rabbit pAb
Catalog No. A6121
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-405 of human Cystathionase/Cystathionase/CTH (NP_001893.2).
Sequence MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGHSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS
Gene ID 1491
Swiss prot P32929
Synonyms CGL; CSE; Cystathionase/CTH
Calculated MW 45kDa
Observed MW 45kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples K-562, Mouse liver, Mouse kidney, Rat liver, Rat kidney
Cellular location Cytoplasm
Customer validation

WB (Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Cystathionase/CTH Rabbit pAb images

ABclonal:Western blot - Cystathionase/CTH Rabbit pAb (A6121)}

Western blot - Cystathionase/CTH Rabbit pAb (A6121)

Western blot analysis of various lysates, using Cystathionase/CTH Rabbit pAb (A6121) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - Cystathionase/CTH Rabbit pAb (A6121)}

Immunofluorescence - Cystathionase/CTH Rabbit pAb (A6121)

Immunofluorescence analysis of HepG2 cells using Cystathionase/CTH Rabbit pAb (A6121) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Cystathionase/CTH Rabbit pAb (A6121)}

Immunoprecipitation - Cystathionase/CTH Rabbit pAb (A6121)

Immunoprecipitation analysis of 300 μg extracts of K-562 cells using 3 μg Cystathionase/CTH antibody (A6121). Western blot was performed from the immunoprecipitate using Cystathionase/CTH antibody (A6121) at a dilution of 1:500.

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Related Targets

Based on existing publications, the following genes are closely related to research on CTH. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CTH. (Distance between topics and target gene indicate popularity.) CTH

* Data provided by citexs.com, for reference only.

Publishing research using A6121? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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