Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

COX5A Rabbit pAb (A6437)

Publications (7) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - COX5A Rabbit pAb (A6437)

Western blot analysis of extracts of various cell lines, using COX5A Antibody (A6437) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - COX5A Rabbit pAb (A6437)

Immunohistochemistry analysis of paraffin-embedded rat ovary using COX5A antibody (A6437) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - COX5A Rabbit pAb (A6437)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using COX5A antibody (A6437) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - COX5A Rabbit pAb (A6437)

Immunohistochemistry analysis of paraffin-embedded mouse testis using COX5A antibody (A6437) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - COX5A Rabbit pAb (A6437)

Immunofluorescence analysis of U2OS cells using COX5A antibody (A6437). Blue: DAPI for nuclear staining.

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Overview

Product name COX5A Rabbit pAb
Catalog No. A6437
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human COX5A (NP_004246.2).
Sequence MLGAALRRCAVAATTRADPRGLLHSARTPGPAVAIQSVRCYSHGSQETDEEFDARWVTYFNKPDIDAWELRKGINTLVTYDMVPEPKIIDAALRACRRLNDFASTVRILEVVKDKAGPHKEIYPYVIQELRPTLNELGISTPEELGLDKV
Gene ID 9377
Swiss prot P20674
Synonyms VA; COX; COX-VA; MC4DN20; COX5A
Calculated MW 17kDa
Observed MW 14kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:10 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, HepG2, mouse skeletal muscle, mouse testis, mouse heart, mouse brain, rat heart, rat kidney, Jurkat
Cellular location Mitochondrion inner membrane
Customer validation

WB (Mus musculus, Homo sapiens, Gallus gallus)

IHC (Homo sapiens)

IB (Mus musculus)

IP (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

COX5A Rabbit pAb images

ABclonal:Western blot - COX5A Rabbit pAb (A6437)}

Western blot - COX5A Rabbit pAb (A6437)

Western blot analysis of extracts of various cell lines, using COX5A Antibody (A6437) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - COX5A Rabbit pAb (A6437)}

Immunohistochemistry - COX5A Rabbit pAb (A6437)

Immunohistochemistry analysis of paraffin-embedded rat ovary using COX5A antibody (A6437) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - COX5A Rabbit pAb (A6437)}

Immunohistochemistry - COX5A Rabbit pAb (A6437)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using COX5A antibody (A6437) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - COX5A Rabbit pAb (A6437)}

Immunohistochemistry - COX5A Rabbit pAb (A6437)

Immunohistochemistry analysis of paraffin-embedded mouse testis using COX5A antibody (A6437) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - COX5A Rabbit pAb (A6437)}

Immunofluorescence - COX5A Rabbit pAb (A6437)

Immunofluorescence analysis of U2OS cells using COX5A antibody (A6437). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on COX5A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to COX5A. (Distance between topics and target gene indicate popularity.) COX5A

* Data provided by citexs.com, for reference only.

Publishing research using A6437? Please let us know so that we can cite the reference in this datasheet.

ELISA Kits (1)

Antibodies (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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