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COLQ Polyclonal Antibody (A10448)

Datasheet

ABclonal:Western blot - COLQ Polyclonal Antibody (A10448)

Overview

Product nameCOLQ Polyclonal Antibody
Catalog No.A10448
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the subunit of a collagen-like molecule associated with acetylcholinesterase in skeletal muscle. Each molecule is composed of three identical subunits. Each subunit contains a proline-rich attachment domain (PRAD) that binds an acetylcholinesterase tetramer to anchor the catalytic subunit of the enzyme to the basal lamina. Mutations in this gene are associated with endplate acetylcholinesterase deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 276-455 of human COLQ (NP_005668.2).
SequenceMGPKGERGFPGPPGRCLCGPTMNVNNPSYGESVYGPSSPRVPVIFVVNNQEELERLNTQNAIAFRRDQRSLYFKDSLGWLPIQLTPFYPVDYTADQHGTCGDGLLQPGEECDDGNSDVGDDCIRCHRAYCGDGHRHEGVEDCDGSDFGYLTCETYLPGSYGDLQCTQYCYIDSTPCRYFT
Gene ID8292
Swiss protQ9Y215
SynonymsCOLQ; CMS5; EAD; acetylcholinesterase collagenic tail peptide
Calculated MW28kDa/30kDa/33kDa/44kDa/46kDa/47kDa
Observed MW48kDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse skeletal muscle, Mouse lung, Rat skeletal muscle, Rat heart, Rat testis
Cellular locationCell junction, synapse
ABclonal:Western blot - COLQ Polyclonal Antibody (A10448) }

Western blot - COLQ Polyclonal Antibody (A10448)

Western blot analysis of extracts of various cell lines, using COLQ antibody (A10448) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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