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COL1A2 Rabbit pAb (A5786)

Publications (5) Datasheet

ABclonal:Western blot - COL1A2 Rabbit pAb (A5786)

Western blot analysis of extracts of various cell lines, using COL1A2 antibody (A5786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - COL1A2 Rabbit pAb (A5786)

Immunohistochemistry of paraffin-embedded human placenta using COL1A2 Rabbit pAb (A5786) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - COL1A2 Rabbit pAb (A5786)

Immunofluorescence analysis of A-431 cells using COL1A2 antibody (A5786) at dilution of 1:25. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - COL1A2 Rabbit pAb (A5786)

Immunofluorescence analysis of NIH/3T3 cells using COL1A2 antibody (A5786) at dilution of 1:25. Blue: DAPI for nuclear staining.

Overview

Product nameCOL1A2 Rabbit pAb
Catalog No.A5786
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 500-600 of human COL1A2 (NP_000080.2).
SequencePTGDPGKNGDKGHAGLAGARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPPGPPGFQGLPGPSGPAGEVGKPGERGLHGEFGLPGPAGPRGERGPPGESGAA
Gene ID1278
Swiss protP08123
SynonymsCOL1A2; OI4
Calculated MW129kDa
Observed MW129KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:25 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse skin, Rat skin
Cellular locationSecreted, extracellular matrix, extracellular space
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

ABclonal:Western blot - COL1A2 Rabbit pAb (A5786) }

Western blot - COL1A2 Rabbit pAb (A5786)

Western blot analysis of extracts of various cell lines, using COL1A2 antibody (A5786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - COL1A2 Rabbit pAb (A5786) }

Immunohistochemistry - COL1A2 Rabbit pAb (A5786)

Immunohistochemistry of paraffin-embedded human placenta using COL1A2 Rabbit pAb (A5786) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - COL1A2 Rabbit pAb (A5786) }

Immunofluorescence - COL1A2 Rabbit pAb (A5786)

Immunofluorescence analysis of A-431 cells using COL1A2 antibody (A5786) at dilution of 1:25. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - COL1A2 Rabbit pAb (A5786) }

Immunofluorescence - COL1A2 Rabbit pAb (A5786)

Immunofluorescence analysis of NIH/3T3 cells using COL1A2 antibody (A5786) at dilution of 1:25. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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