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CNN1 Rabbit pAb (A16638)

Datasheet

ABclonal:Western blot - CNN1 Polyclonal Antibody (A16638)

Overview

Product nameCNN1 Rabbit pAb
Catalog No.A16638
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 130-180 of human CNN1 (NP_001290.2).
SequenceMAKTKGNKVNVGVKYAEKQERKFEPGKLREGRNIIGLQMGTNKFASQQGMT
Gene ID1264
Swiss protP51911
SynonymsCNN1; HEL-S-14; SMCC; Sm-Calp
Calculated MW31kDa/33kDa
Observed MW33kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-87MG, PC-3, HeLa, Mouse spleen, Rat testis
Cellular location
ABclonal:Western blot - CNN1 Polyclonal Antibody (A16638) }

Western blot - CNN1 Polyclonal Antibody (A16638)

Western blot analysis of extracts of various cell lines, using CNN1 antibody (A16638) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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