Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CNBP Rabbit pAb (A15110)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CNBP Rabbit pAb (A15110)

Western blot analysis of various lysates, using CNBP Rabbit pAb (A15110) at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - CNBP Rabbit pAb (A15110)

Immunohistochemistry analysis of CNBP in paraffin-embedded Human kidney tissue using CNBP Rabbit pAb (A15110) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CNBP Rabbit pAb (A15110)

Immunohistochemistry analysis of CNBP in paraffin-embedded Human liver tissue using CNBP Rabbit pAb (A15110) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CNBP Rabbit pAb (A15110)

Immunohistochemistry analysis of CNBP in paraffin-embedded Mouse colon tissue using CNBP Rabbit pAb (A15110) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CNBP Rabbit pAb (A15110)

Immunohistochemistry analysis of CNBP in paraffin-embedded Rat colon tissue using CNBP Rabbit pAb (A15110) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CNBP Rabbit pAb (A15110)

Immunofluorescence analysis of U-2 OS cells using CNBP Rabbit pAb (A15110) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name CNBP Rabbit pAb
Catalog No. A15110
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion from <30 repeats to 75-11000 repeats in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 70-170 of human CNBP (NP_001120668.1).
Sequence CGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCDHADEQKCYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTIEATA
Gene ID 7555
Swiss prot P62633
Synonyms DM2; ZNF9; CNBP1; PROMM; RNF163; ZCCHC22; CNBP
Calculated MW 19kDa
Observed MW 20kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, 293F, NIH/3T3
Cellular location Nucleus, Cytoplasm, Endoplasmic reticulum
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

CNBP Rabbit pAb images

ABclonal:Western blot - CNBP Rabbit pAb (A15110)}

Western blot - CNBP Rabbit pAb (A15110)

Western blot analysis of various lysates, using CNBP Rabbit pAb (A15110) at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - CNBP Rabbit pAb (A15110)}

Immunohistochemistry - CNBP Rabbit pAb (A15110)

Immunohistochemistry analysis of CNBP in paraffin-embedded Human kidney tissue using CNBP Rabbit pAb (A15110) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CNBP Rabbit pAb (A15110)}

Immunohistochemistry - CNBP Rabbit pAb (A15110)

Immunohistochemistry analysis of CNBP in paraffin-embedded Human liver tissue using CNBP Rabbit pAb (A15110) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CNBP Rabbit pAb (A15110)}

Immunohistochemistry - CNBP Rabbit pAb (A15110)

Immunohistochemistry analysis of CNBP in paraffin-embedded Mouse colon tissue using CNBP Rabbit pAb (A15110) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CNBP Rabbit pAb (A15110)}

Immunohistochemistry - CNBP Rabbit pAb (A15110)

Immunohistochemistry analysis of CNBP in paraffin-embedded Rat colon tissue using CNBP Rabbit pAb (A15110) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - CNBP Rabbit pAb (A15110)}

Immunofluorescence - CNBP Rabbit pAb (A15110)

Immunofluorescence analysis of U-2 OS cells using CNBP Rabbit pAb (A15110) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on CNBP. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to CNBP. (Distance between topics and target gene indicate popularity.) CNBP

* Data provided by citexs.com, for reference only.

Publishing research using A15110? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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