Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CKAP5 Rabbit pAb (A8532)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CKAP5 Rabbit pAb (A8532)

Western blot analysis of various lysates using CKAP5 Rabbit pAb (A8532) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - CKAP5 Rabbit pAb (A8532)

Immunofluorescence analysis of U2OS cells using CKAP5 Rabbit pAb (A8532) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CKAP5 Rabbit pAb (A8532)

Immunofluorescence analysis of paraffin-embedded Rat H9C2 using CKAP5 Rabbit pAb (A8532) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CKAP5 Rabbit pAb (A8532)

Immunofluorescence analysis of paraffin-embedded Mouse L929 using CKAP5 Rabbit pAb (A8532) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CKAP5 Rabbit pAb (A8532)

Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 3 μg CKAP5 antibody (A8532). Western blot was performed from the immunoprecipitate using CKAP5 antibody (A8532) at a dilution of 1:1000.

You may also interested in:

Overview

Product name CKAP5 Rabbit pAb
Catalog No. A8532
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1763-2032 of human CKAP5 (NP_001008938.1).
Sequence LLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRISTSTGISPQMEVTCVPTPTSTVSSIGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTHSSGTVTSSSSTANIDDLKKRLERIKSSRK
Gene ID 9793
Swiss prot Q14008
Synonyms TOG; MSPS; TOGp; CHTOG; ch-TOG; CKAP5
Calculated MW 225kDa
Observed MW 250kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples HeLa, Jurkat, HepG2, HT-29, Mouse brain, Mouse lung, Mouse liver, Rat brain
Cellular location Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle pole

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CKAP5 Rabbit pAb images

ABclonal:Western blot - CKAP5 Rabbit pAb (A8532)}

Western blot - CKAP5 Rabbit pAb (A8532)

Western blot analysis of various lysates using CKAP5 Rabbit pAb (A8532) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - CKAP5 Rabbit pAb (A8532)}

Immunofluorescence - CKAP5 Rabbit pAb (A8532)

Immunofluorescence analysis of U2OS cells using CKAP5 Rabbit pAb (A8532) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CKAP5 Rabbit pAb (A8532)}

Immunofluorescence - CKAP5 Rabbit pAb (A8532)

Immunofluorescence analysis of paraffin-embedded Rat H9C2 using CKAP5 Rabbit pAb (A8532) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CKAP5 Rabbit pAb (A8532)}

Immunofluorescence - CKAP5 Rabbit pAb (A8532)

Immunofluorescence analysis of paraffin-embedded Mouse L929 using CKAP5 Rabbit pAb (A8532) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - CKAP5 Rabbit pAb (A8532)}

Immunoprecipitation - CKAP5 Rabbit pAb (A8532)

Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 3 μg CKAP5 antibody (A8532). Western blot was performed from the immunoprecipitate using CKAP5 antibody (A8532) at a dilution of 1:1000.

Inquire About This Product

Submit your question about A8532 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on CKAP5. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CKAP5. (Distance between topics and target gene indicate popularity.) CKAP5

* Data provided by citexs.com, for reference only.

Publishing research using A8532? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order