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CIAPIN1 Rabbit pAb (A6336)

Datasheet

ABclonal:Western blot - CIAPIN1 Polyclonal Antibody (A6336)
ABclonal:Immunohistochemistry - CIAPIN1 Polyclonal Antibody (A6336)
ABclonal:Immunohistochemistry - CIAPIN1 Polyclonal Antibody (A6336)
ABclonal:Immunohistochemistry - CIAPIN1 Polyclonal Antibody (A6336)
ABclonal:Immunofluorescence - CIAPIN1 Polyclonal Antibody (A6336)
ABclonal:Immunoprecipitation - CIAPIN1 Polyclonal Antibody (A6336)

Overview

Product nameCIAPIN1 Rabbit pAb
Catalog No.A6336
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human CIAPIN1 (NP_064709.2).
SequenceMADFGISAGQFVAVVWDKSSPVEALKGLVDKLQALTGNEGRVSVENIKQLLQSAHKESSFDIILSGLVPGSTTLHSAEILAEIARILRPGGCLFLKEPVETAVDNNSKVKTASKLCSALTLSGLVEVKELQREPLTPEEVQSVREHLGHESDNLLFVQITGKKPNFEVGSSRQLKLSITKKSSPSVKPAVDPAAAKLWTLSANDMEDDSMDLIDSDELLDPEDLKKPDPASLRAASCGEGKKRKACKNCTCGLAEELEKEKSREQMSSQPKSACGNCYLGDAFRCASCPYLGMPAFKPGEKVLLSDSNLHDA
Gene ID57019
Swiss protQ6FI81
SynonymsCIAPIN1; Anamorsin; DRE2; PRO0915; anamorsin
Calculated MW11kDa/32kDa/33kDa
Observed MW39kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesSKOV3, 293T, HepG2, HeLa, MCF-7, Mouse testis
Cellular locationCytoplasm, Mitochondrion, Mitochondrion intermembrane space, Nucleus
ABclonal:Western blot - CIAPIN1 Polyclonal Antibody (A6336) }

Western blot - CIAPIN1 Polyclonal Antibody (A6336)

Western blot analysis of extracts of various cell lines, using CIAPIN1 antibody (A6336) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - CIAPIN1 Polyclonal Antibody (A6336) }

Immunohistochemistry - CIAPIN1 Polyclonal Antibody (A6336)

Immunohistochemistry of paraffin-embedded human liver damage using CIAPIN1 Antibody (A6336) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CIAPIN1 Polyclonal Antibody (A6336) }

Immunohistochemistry - CIAPIN1 Polyclonal Antibody (A6336)

Immunohistochemistry of paraffin-embedded human gastric cancer using CIAPIN1 Antibody (A6336) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CIAPIN1 Polyclonal Antibody (A6336) }

Immunohistochemistry - CIAPIN1 Polyclonal Antibody (A6336)

Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using CIAPIN1 Antibody (A6336) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CIAPIN1 Polyclonal Antibody (A6336) }

Immunofluorescence - CIAPIN1 Polyclonal Antibody (A6336)

Immunofluorescence analysis of U2OS cells using CIAPIN1 antibody (A6336). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - CIAPIN1 Polyclonal Antibody (A6336) }

Immunoprecipitation - CIAPIN1 Polyclonal Antibody (A6336)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug CIAPIN1 antibody (A6336). Western blot was performed from the immunoprecipitate using CIAPIN1 antibody (A6336) at a dilition of 1:1000.

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