Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CHCHD3 Rabbit pAb (A8584)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CHCHD3 Rabbit pAb (A8584)

Western blot analysis of various lysates using CHCHD3 Rabbit pAb (A8584) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.

ABclonal:Immunofluorescence - CHCHD3 Rabbit pAb (A8584)

Confocal immunofluorescence analysis of U2OS cells using CHCHD3 Rabbit pAb (A8584) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CHCHD3 Rabbit pAb (A8584)

Immunoprecipitation analysis of 600 μg extracts of Mouse liver cells using 3 μg CHCHD3 antibody (A8584). Western blot was performed from the immunoprecipitate using CHCHD3 antibody (A8584) at a dilution of 1:1000.

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Overview

Product name CHCHD3 Rabbit pAb
Catalog No. A8584
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human CHCHD3 (NP_060282.1).
Sequence MGGTTSTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAKELDRERAAANEQLTRAILRERICSEEERAKAKHLARQLEEKDRVLKKQDAFYKEQLARLEERSSEFYRVTTEQYQKAAEEVEAKFKRYESHPVCADLQAKILQCYRENTHQTLKCSALATQYMHCVNHAKQSMLEKGG
Gene ID 54927
Swiss prot Q9NX63
Synonyms Mic19; MINOS3; MICOS19; PPP1R22; CHCHD3
Calculated MW 26kDa
Observed MW 30kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples A-549, HeLa, A-431, Jurkat, HepG2, mouse kidney, mouse liver, rat heart, rat kidney, Mouse kidney, Mouse liver, Mouse heart
Cellular location Cytoplasm, Intermembrane side, Lipid-anchor, Mitochondrion, Mitochondrion inner membrane, Nucleus
Customer validation

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CHCHD3 Rabbit pAb images

ABclonal:Western blot - CHCHD3 Rabbit pAb (A8584)}

Western blot - CHCHD3 Rabbit pAb (A8584)

Western blot analysis of various lysates using CHCHD3 Rabbit pAb (A8584) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.
ABclonal:Immunofluorescence - CHCHD3 Rabbit pAb (A8584)}

Immunofluorescence - CHCHD3 Rabbit pAb (A8584)

Confocal immunofluorescence analysis of U2OS cells using CHCHD3 Rabbit pAb (A8584) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - CHCHD3 Rabbit pAb (A8584)}

Immunoprecipitation - CHCHD3 Rabbit pAb (A8584)

Immunoprecipitation analysis of 600 μg extracts of Mouse liver cells using 3 μg CHCHD3 antibody (A8584). Western blot was performed from the immunoprecipitate using CHCHD3 antibody (A8584) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on CHCHD3. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to CHCHD3. (Distance between topics and target gene indicate popularity.) CHCHD3

* Data provided by citexs.com, for reference only.

Publishing research using A8584? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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