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CDH11 Rabbit pAb (A8110)

Publications (2) Datasheet

ABclonal:Western blot - CDH11 Polyclonal Antibody (A8110)

Western blot analysis of extracts of various cell lines, using CDH11 antibody (A8110) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - CDH11 Polyclonal Antibody (A8110)

Immunohistochemistry of paraffin-embedded mouse brain using CDH11 antibody (A8110) at dilution of 1:100 (40x lens).

Overview

Product nameCDH11 Rabbit pAb
Catalog No.A8110
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 637-796 of human CDH11 (NP_001788.2).
SequenceFVTLRRQKKEPLIVFEEEDVRENIITYDDEGGGEEDTEAFDIATLQNPDGINGFIPRKDIKPEYQYMPRPGLRPAPNSVDVDDFINTRIQEADNDPTAPPYDSIQIYGYEGRGSVAGSLSSLESATTDSDLDYDYLQNWGPRFKKLADLYGSKDTFDDDS
Gene ID1009
Swiss protP55287
SynonymsCDH11; CAD11; CDHOB; OB; OSF-4
Calculated MW76kDa/87kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, A-549, HT-29, SKOV3, Mouse lung, Mouse spleen
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)

ABclonal:Western blot - CDH11 Polyclonal Antibody (A8110) }

Western blot - CDH11 Polyclonal Antibody (A8110)

Western blot analysis of extracts of various cell lines, using CDH11 antibody (A8110) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CDH11 Polyclonal Antibody (A8110) }

Immunohistochemistry - CDH11 Polyclonal Antibody (A8110)

Immunohistochemistry of paraffin-embedded mouse brain using CDH11 antibody (A8110) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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