Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TCP1 beta/CCT2 Rabbit pAb (A6546)

Publications (3) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - TCP1 beta/CCT2 Rabbit pAb (A6546)

Western blot analysis of various lysates using TCP1 beta/CCT2 Rabbit pAb (A6546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunofluorescence - TCP1 beta/CCT2 Rabbit pAb (A6546)

Immunofluorescence analysis of HeLa cells using TCP1 beta/CCT2 Rabbit pAb (A6546). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - TCP1 beta/CCT2 Rabbit pAb (A6546)

Immunoprecipitation analysis of 200 μg extracts of A-549 cells, using 3 μg TCP1 beta/TCP1 beta/CCT2 antibody (A6546). Western blot was performed from the immunoprecipitate using TCP1 beta/TCP1 beta/CCT2 antibody (A6546) at a dilution of 1:1000.

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Overview

Product name TCP1 beta/CCT2 Rabbit pAb
Catalog No. A6546
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 206-535 of human TCP1 beta/TCP1 beta/CCT2 (NP_006422.1).
Sequence GGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC
Gene ID 10576
Swiss prot P78371
Synonyms CCTB; 99D8.1; PRO1633; CCT-beta; HEL-S-100n; TCP-1-beta; TCP1 beta/CCT2
Calculated MW 57kDa
Observed MW 57kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples MCF7, SKOV3, A-549, HT-29, Mouse spleen, Mouse ovary, Mouse heart
Cellular location Cytoplasm
Customer validation

WB (Rattus norvegicus, Homo sapiens, Mus musculus)

IF (Homo sapiens, Mus musculus)

DB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

TCP1 beta/CCT2 Rabbit pAb images

ABclonal:Western blot - TCP1 beta/CCT2 Rabbit pAb (A6546)}

Western blot - TCP1 beta/CCT2 Rabbit pAb (A6546)

Western blot analysis of various lysates using TCP1 beta/CCT2 Rabbit pAb (A6546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunofluorescence - TCP1 beta/CCT2 Rabbit pAb (A6546)}

Immunofluorescence - TCP1 beta/CCT2 Rabbit pAb (A6546)

Immunofluorescence analysis of HeLa cells using TCP1 beta/CCT2 Rabbit pAb (A6546). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - TCP1 beta/CCT2 Rabbit pAb (A6546)}

Immunoprecipitation - TCP1 beta/CCT2 Rabbit pAb (A6546)

Immunoprecipitation analysis of 200 μg extracts of A-549 cells, using 3 μg TCP1 beta/TCP1 beta/CCT2 antibody (A6546). Western blot was performed from the immunoprecipitate using TCP1 beta/TCP1 beta/CCT2 antibody (A6546) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on CCT2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCT2. (Distance between topics and target gene indicate popularity.) CCT2

* Data provided by citexs.com, for reference only.

Publishing research using A6546? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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