Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Cyclin K Rabbit pAb (A10261)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Cyclin K Rabbit pAb (A10261)

Western blot analysis of extracts of various cell lines, using Cyclin K Antibody (A10261) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Cyclin K Rabbit pAb (A10261)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using Cyclin K Rabbit pAb (A10261) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Cyclin K Rabbit pAb (A10261)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Cyclin K Rabbit pAb (A10261) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Cyclin K Rabbit pAb (A10261)

Immunohistochemistry analysis of paraffin-embedded rat brain using Cyclin K Rabbit pAb (A10261) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

You may also interested in:

Overview

Product name Cyclin K Rabbit pAb
Catalog No. A10261
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the transcription cyclin family. These cyclins may regulate transcription through their association with and activation of cyclin-dependent kinases (CDK) that phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II. This gene product may play a dual role in regulating CDK and RNA polymerase II activities.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human Cyclin K (NP_001092872.1).
Sequence MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDTLATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHP
Gene ID 8812
Swiss prot O75909
Synonyms CPR4; IDDHDF; Cyclin K
Calculated MW 64kDa
Observed MW 90kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa, Mouse brain
Cellular location Nucleus
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Cyclin K Rabbit pAb images

ABclonal:Western blot - Cyclin K Rabbit pAb (A10261)}

Western blot - Cyclin K Rabbit pAb (A10261)

Western blot analysis of extracts of various cell lines, using Cyclin K Antibody (A10261) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Cyclin K Rabbit pAb (A10261)}

Immunohistochemistry - Cyclin K Rabbit pAb (A10261)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using Cyclin K Rabbit pAb (A10261) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Cyclin K Rabbit pAb (A10261)}

Immunohistochemistry - Cyclin K Rabbit pAb (A10261)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Cyclin K Rabbit pAb (A10261) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Cyclin K Rabbit pAb (A10261)}

Immunohistochemistry - Cyclin K Rabbit pAb (A10261)

Immunohistochemistry analysis of paraffin-embedded rat brain using Cyclin K Rabbit pAb (A10261) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Inquire About This Product

Submit your question about A10261 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on CCNK. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCNK. (Distance between topics and target gene indicate popularity.) CCNK

* Data provided by citexs.com, for reference only.

Publishing research using A10261? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order