Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Cyclin E1 Rabbit pAb (A0112)

Reviews (3) Publications (6) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Cyclin E1 Rabbit pAb (A0112)

Western blot analysis of extracts of A2058 cells, using Cyclin E1 antibody (A0112).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Review
ABclonal: review for Cyclin E1 Rabbit pAb(A0112)
Review
ABclonal: review for Cyclin E1 Rabbit pAb(A0112)
Review
ABclonal: review for Cyclin E1 Rabbit pAb(A0112)

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Overview

Product name Cyclin E1 Rabbit pAb
Catalog No. A0112
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

Immunogen information

Immunogen A synthetic peptide of human Cyclin E1
Sequence Email for sequence
Gene ID 898
Swiss prot P24864
Synonyms CCNE; pCCNE1; Cyclin E1
Calculated MW 47kDa
Observed MW 50kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • FC 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Flow Cytometry    
Positive samples A2058
Cellular location Nucleus
Customer validation

WB (H460,A549, HeLaHomo sapiens, Homo sapiens, Rattus norvegicus)

IP (Rattus norvegicus)

Research Area

Cyclin E1 Rabbit pAb images

ABclonal:Western blot - Cyclin E1 Rabbit pAb (A0112)}

Western blot - Cyclin E1 Rabbit pAb (A0112)

Western blot analysis of extracts of A2058 cells, using Cyclin E1 antibody (A0112).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on CCNE1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCNE1. (Distance between topics and target gene indicate popularity.) CCNE1

* Data provided by citexs.com, for reference only.

Publishing research using A0112? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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