Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[KO Validated] Cyclin B1 Rabbit pAb (A16800)

KO/KDValidated

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Cyclin B1 Rabbit pAb (A16800)

Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit pAb (A16800) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - [KO Validated] Cyclin B1 Rabbit pAb (A16800)

Immunohistochemistry analysis of Cyclin B1 in paraffin-embedded human placenta tissue using [KO Validated] Cyclin B1 Rabbit pAb (A16800) at a dilution of 1:150 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] Cyclin B1 Rabbit pAb (A16800)

Immunohistochemistry analysis of Cyclin B1 in paraffin-embedded rat ovary tissue using [KO Validated] Cyclin B1 Rabbit pAb (A16800) at a dilution of 1:150 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] Cyclin B1 Rabbit pAb (A16800)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Cyclin B1 Rabbit pAb (A16800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] Cyclin B1 Rabbit pAb (A16800)

Immunofluorescence analysis of PC-12 cells using [KO Validated] Cyclin B1 Rabbit pAb (A16800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name [KO Validated] Cyclin B1 Rabbit pAb
Catalog No. A16800
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 350-433 of human CCNB1 (NP_114172.1).
Sequence CLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV
Gene ID 891
Swiss prot P14635
Synonyms CCNB; B1
Calculated MW 48kDa
Observed MW 55kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa
Cellular location Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] Cyclin B1 Rabbit pAb images

ABclonal:Western blot - [KO Validated] Cyclin B1 Rabbit pAb (A16800)}

Western blot - [KO Validated] Cyclin B1 Rabbit pAb (A16800)

Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit pAb (A16800) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - [KO Validated] Cyclin B1 Rabbit pAb (A16800)}

Immunohistochemistry - [KO Validated] Cyclin B1 Rabbit pAb (A16800)

Immunohistochemistry analysis of Cyclin B1 in paraffin-embedded human placenta tissue using [KO Validated] Cyclin B1 Rabbit pAb (A16800) at a dilution of 1:150 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] Cyclin B1 Rabbit pAb (A16800)}

Immunohistochemistry - [KO Validated] Cyclin B1 Rabbit pAb (A16800)

Immunohistochemistry analysis of Cyclin B1 in paraffin-embedded rat ovary tissue using [KO Validated] Cyclin B1 Rabbit pAb (A16800) at a dilution of 1:150 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] Cyclin B1 Rabbit pAb (A16800)}

Immunofluorescence - [KO Validated] Cyclin B1 Rabbit pAb (A16800)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Cyclin B1 Rabbit pAb (A16800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Cyclin B1 Rabbit pAb (A16800)}

Immunofluorescence - [KO Validated] Cyclin B1 Rabbit pAb (A16800)

Immunofluorescence analysis of PC-12 cells using [KO Validated] Cyclin B1 Rabbit pAb (A16800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on CCNB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCNB1. (Distance between topics and target gene indicate popularity.) CCNB1

* Data provided by citexs.com, for reference only.

Publishing research using A16800? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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