Products > Catalog Antibodies > Polyclonal Antibodies

CCNA2 Polyclonal Antibody (A7632)

Publications (2) Datasheet

ABclonal:Western blot - CCNA2 antibody (A7632)
ABclonal:Immunohistochemistry - CCNA2 antibody (A7632)
ABclonal:Immunohistochemistry - CCNA2 antibody (A7632)
ABclonal:Immunofluorescence - CCNA2 antibody (A7632)

Overview

Product nameCCNA2 Polyclonal Antibody
Catalog No.A7632
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
ImmunogenA synthetic peptide of human CCNA2
SequenceEmail for sequence
Gene ID890
Swiss protP20248
SynonymsCCN1; CCNA
Calculated MW48kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Mouse liver, Mouse spleen, Mouse eye, Rat spinal cord
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Rat)

IP(Rat)

ABclonal:Western blot - CCNA2 antibody (A7632)}

Western blot - CCNA2 antibody (A7632)

Western blot analysis of extracts of various cell lines, using CCNA2 antibody (A7632) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CCNA2 antibody (A7632)}

Immunohistochemistry - CCNA2 antibody (A7632)

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using CCNA2 antibody (A7632) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CCNA2 antibody (A7632)}

Immunohistochemistry - CCNA2 antibody (A7632)

Immunohistochemistry of paraffin-embedded mouse kidney using CCNA2 antibody (A7632) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CCNA2 antibody (A7632)}

Immunofluorescence - CCNA2 antibody (A7632)

Immunofluorescence analysis of U2OS cells using CCNA2 antibody (A7632) at dilution of 1:100. Blue: DAPI for nuclear staining.

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