Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

CCL2/MCP-1 Rabbit mAb (A22744)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - CCL2/MCP-1 Rabbit mAb (A22744)

Western blot analysis of lysates from A-549 cells, using CCL2/MCP-1 Rabbit mAb (A22744) at 1:8000 dilution.A-549 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Overview

Product name CCL2/MCP-1 Rabbit mAb
Catalog No. A22744
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC58653

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. Elevated expression of the encoded protein is associated with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 22-99 of human CCL2/MCP-1(NP_002973.1).
Sequence LAQPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT
Gene ID 6347
Swiss prot P13500
Synonyms HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30; CCL2/MCP-1
Calculated MW 11kDa
Observed MW 13-15kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:9000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples A549+TNF-α
Cellular location Secreted

Documents

Certificate of Compliance

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Lot number

Research Area

CCL2/MCP-1 Rabbit mAb images

ABclonal:Western blot - CCL2/MCP-1 Rabbit mAb (A22744)}

Western blot - CCL2/MCP-1 Rabbit mAb (A22744)

Western blot analysis of lysates from A-549 cells, using CCL2/MCP-1 Rabbit mAb (A22744) at 1:8000 dilution.A-549 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CCL2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCL2. (Distance between topics and target gene indicate popularity.) CCL2

* Data provided by citexs.com, for reference only.

Publishing research using A22744? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Proteins (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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