Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CCAR2 Rabbit pAb (A7126)

Review (1) Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CCAR2 Rabbit pAb (A7126)

Western blot analysis of extracts of various cell lines, using CCAR2 antibody (A7126) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - CCAR2 Rabbit pAb (A7126)

Immunohistochemistry analysis of paraffin-embedded rat brain using CCAR2 antibody (A7126) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CCAR2 Rabbit pAb (A7126)

Immunohistochemistry analysis of paraffin-embedded human rectal cancer using CCAR2 antibody (A7126) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CCAR2 Rabbit pAb (A7126)

Immunohistochemistry analysis of paraffin-embedded mouse brain using CCAR2 antibody (A7126) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Review
ABclonal: review for CCAR2 Rabbit pAb(A7126)

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Overview

Product name CCAR2 Rabbit pAb
Catalog No. A7126
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables RNA polymerase II complex binding activity and enzyme inhibitor activity. Involved in several processes, including regulation of cellular protein metabolic process; regulation of signal transduction; and regulation of transcription, DNA-templated. Located in several cellular components, including mitochondrial matrix; nucleoplasm; and spindle. Part of DBIRD complex.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 654-923 of human CCAR2 (NP_066997.3).
Sequence LLRDDGEEEFAGAKLEDSEVRSVASNQSEMEFSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVTQNICQYRSLQYSRQEGLDGGLPEEVLFGNLDLLPPPGKSTKPGAAPTEHKALVSHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRVRLAEAEETARTAERQKSQLQRLLQELRRRLTPLQLEIQRVVEKADSWVEKEEPAPSN
Gene ID 57805
Swiss prot Q8N163
Synonyms DBC1; DBC-1; NET35; p30DBC; p30 DBC; KIAA1967; CCAR2
Calculated MW 103kDa
Observed MW 130kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Jurkat, HeLa, Rat lung
Cellular location Cytoplasm, Nucleus
Customer validation

WB (A172)

CHIP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CCAR2 Rabbit pAb images

ABclonal:Western blot - CCAR2 Rabbit pAb (A7126)}

Western blot - CCAR2 Rabbit pAb (A7126)

Western blot analysis of extracts of various cell lines, using CCAR2 antibody (A7126) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - CCAR2 Rabbit pAb (A7126)}

Immunohistochemistry - CCAR2 Rabbit pAb (A7126)

Immunohistochemistry analysis of paraffin-embedded rat brain using CCAR2 antibody (A7126) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CCAR2 Rabbit pAb (A7126)}

Immunohistochemistry - CCAR2 Rabbit pAb (A7126)

Immunohistochemistry analysis of paraffin-embedded human rectal cancer using CCAR2 antibody (A7126) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CCAR2 Rabbit pAb (A7126)}

Immunohistochemistry - CCAR2 Rabbit pAb (A7126)

Immunohistochemistry analysis of paraffin-embedded mouse brain using CCAR2 antibody (A7126) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on CCAR2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCAR2. (Distance between topics and target gene indicate popularity.) CCAR2

* Data provided by citexs.com, for reference only.

Publishing research using A7126? Please let us know so that we can cite the reference in this datasheet.

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