Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CBX4 Rabbit pAb (A5532)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CBX4 Rabbit pAb (A5532)

Western blot analysis of various lysates using CBX4 Rabbit pAb (A5532) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunoprecipitation - CBX4 Rabbit pAb (A5532)

Immunoprecipitation analysis of 200 μg extracts of HepG2 cells using 1 μg CBX4 antibody (A5532). Western blot was performed from the immunoprecipitate using CBX4 antibody (A5532) at a dilution of 1:500.

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Overview

Product name CBX4 Rabbit pAb
Catalog No. A5532
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables SUMO binding activity; SUMO ligase activity; and enzyme binding activity. Involved in negative regulation of transcription by RNA polymerase II. Located in nuclear body. Part of PRC1 complex. Implicated in hepatocellular carcinoma. Biomarker of hepatocellular carcinoma.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 401-560 of human CBX4 (NP_003646.2).
Sequence AVGLNLSHVRKRCLSETHGEREPCKKRLTARSISTPTCLGGSPAAERPADLPPAAALPQPEVILLDSDLDEPIDLRCVKTRSEAGEPPSSLQVKPETPASAAVAVAAAAAPTTTAEKPPAEAQDEPAESLSEFKPFFGNIIITDVTANCLTVTFKEYVTV
Gene ID 8535
Swiss prot O00257
Synonyms PC2; NBP16; CBX4
Calculated MW 61kDa
Observed MW 73kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunoprecipitation    
Positive samples MCF7, HepG2, SW620, Jurkat, Mouse brain, Mouse liver, Rat liver
Cellular location Nucleus, Nucleus speckle

Documents

Certificate of Compliance

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Lot number

Research Area

CBX4 Rabbit pAb images

ABclonal:Western blot - CBX4 Rabbit pAb (A5532)}

Western blot - CBX4 Rabbit pAb (A5532)

Western blot analysis of various lysates using CBX4 Rabbit pAb (A5532) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunoprecipitation - CBX4 Rabbit pAb (A5532)}

Immunoprecipitation - CBX4 Rabbit pAb (A5532)

Immunoprecipitation analysis of 200 μg extracts of HepG2 cells using 1 μg CBX4 antibody (A5532). Western blot was performed from the immunoprecipitate using CBX4 antibody (A5532) at a dilution of 1:500.

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Related Targets

Based on existing publications, the following genes are closely related to research on CBX4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CBX4. (Distance between topics and target gene indicate popularity.) CBX4

* Data provided by citexs.com, for reference only.

Publishing research using A5532? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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