Products > Catalog Antibodies > Polyclonal Antibodies

Caspase 9 Polyclonal Antibody (A2636)

Review (1)Publications (4) Datasheet

ABclonal:Western blot - Caspase 9 Polyclonal Antibody (A2636)
ABclonal:Western blot - Caspase 9 Polyclonal Antibody (A2636)
ABclonal:Immunohistochemistry - Caspase 9 Polyclonal Antibody (A2636)
ABclonal:Immunohistochemistry - Caspase 9 Polyclonal Antibody (A2636)
ABclonal:Immunofluorescence - Caspase 9 Polyclonal Antibody (A2636)
Review
ABclonal: review for Caspase 9 Polyclonal Antibody(A2636)

Overview

Product nameCaspase 9 Polyclonal Antibody
Catalog No.A2636
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein of human Caspase 9
SequenceEmail for sequence
Gene ID842
Swiss protP55211
SynonymsCASP9; APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56; caspase-9
Calculated MW17kDa/30kDa/36kDa/46kDa
Observed MW36kDa/46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, K-562, Mouse liver
Cellular location
Customer validation

WB(Human, Mouse)

ABclonal:Western blot - Caspase 9 Polyclonal Antibody (A2636) }

Western blot - Caspase 9 Polyclonal Antibody (A2636)

Western blot analysis of extracts of various cell lines, using Caspase 9 antibody (A2636) at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - Caspase 9 Polyclonal Antibody (A2636) }

Western blot - Caspase 9 Polyclonal Antibody (A2636)

Western blot analysis of extracts of Jurkat cells, using Caspase 9 antibody (A2636) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Caspase 9 Polyclonal Antibody (A2636) }

Immunohistochemistry - Caspase 9 Polyclonal Antibody (A2636)

Immunohistochemistry of paraffin-embedded rat lung using Caspase 9 Antibody (A2636) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Caspase 9 Polyclonal Antibody (A2636) }

Immunohistochemistry - Caspase 9 Polyclonal Antibody (A2636)

Immunohistochemistry of paraffin-embedded rat brain using Caspase 9 Antibody (A2636) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Caspase 9 Polyclonal Antibody (A2636) }

Immunofluorescence - Caspase 9 Polyclonal Antibody (A2636)

Immunofluorescence analysis of A549 cells using Caspase 9 antibody (A2636).

Submit an review for A2636

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A2636 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A2636? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μl sample size available Add to Cart
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order