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Caspase 8 Polyclonal Antibody (A0215)

Reviews (2)Publication (1) Datasheet

ABclonal:Western blot - Caspase 8 Polyclonal Antibody (A0215)
ABclonal:Immunofluorescence - Caspase 8 Polyclonal Antibody (A0215)
ABclonal: review for Caspase 8 Polyclonal Antibody(A0215)
ABclonal: review for Caspase 8 Polyclonal Antibody(A0215)


Product nameCaspase 8 Polyclonal Antibody
Catalog No.A0215
Host speciesRabbit
Purification methodAffinity purification
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-479 of human Caspase 8 (NP_001073593.1).
Gene ID841
Swiss protQ14790
SynonymsCASP8; ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5; caspase-8
Calculated MW25-32kDa/45- 61kDa
Observed MW17kDa/44kDa/60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRaji, HL-60
Cellular locationCytoplasm
Customer validation

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ABclonal:Western blot - Caspase 8 Polyclonal Antibody (A0215) }

Western blot - Caspase 8 Polyclonal Antibody (A0215)

Western blot analysis of extracts of various cell lines, using Caspase 8 antibody (A0215) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunofluorescence - Caspase 8 Polyclonal Antibody (A0215) }

Immunofluorescence - Caspase 8 Polyclonal Antibody (A0215)

Immunofluorescence analysis of NIH/3T3 cells using Caspase 8 antibody (A0215) at dilution of 1:100. Blue: DAPI for nuclear staining.

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