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CASP4 Polyclonal Antibody (A6495)

Publication (1) Datasheet

ABclonal:Western blot - CASP4 Polyclonal Antibody (A6495)

Overview

Product nameCASP4 Polyclonal Antibody
Catalog No.A6495
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.
ImmunogenRecombinant protein of mouse CASP4
SequenceEmail for sequence
Gene ID12363
Swiss prot
SynonymsCASP4; ICE(rel)II; ICEREL-II; ICH-2; Mih1; Mih1/TX; TX; caspase-4
Calculated MW13kDa/18kDa/30kDa/36kDa/43kDa
Observed MW43-48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, HeLa
Cellular locationEndoplasmic reticulum membrane, Mitochondrion
Customer validation

WB(Mouse)

ABclonal:Western blot - CASP4 Polyclonal Antibody (A6495) }

Western blot - CASP4 Polyclonal Antibody (A6495)

Western blot analysis of extracts of various cell lines, using CASP4 antibody (A6495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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