Products > Catalog Antibodies > Polyclonal Antibodies

CASP3 Polyclonal Antibody (A2156)

Reviews (6)Publications (17) Datasheet

ABclonal:Western blot - CASP3 Polyclonal Antibody (A2156)
ABclonal:Western blot - CASP3 Polyclonal Antibody (A2156)
ABclonal:Immunohistochemistry - CASP3 Polyclonal Antibody (A2156)
ABclonal:Immunohistochemistry - CASP3 Polyclonal Antibody (A2156)
ABclonal:Immunohistochemistry - CASP3 Polyclonal Antibody (A2156)
ABclonal:Immunohistochemistry - CASP3 Polyclonal Antibody (A2156)
ABclonal:Immunohistochemistry - CASP3 Polyclonal Antibody (A2156)
ABclonal:Immunofluorescence - CASP3 Polyclonal Antibody (A2156)
ABclonal:Immunofluorescence - CASP3 Polyclonal Antibody (A2156)
ABclonal:Immunofluorescence - CASP3 Polyclonal Antibody (A2156)

Overview

Product nameCASP3 Polyclonal Antibody
Catalog No.A2156
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human CASP3 (NP_004337.2).
SequenceFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFII
Gene ID836
Swiss protP42574
SynonymsCASP3; CPP32; CPP32B; SCA-1; caspase-3
Calculated MW31kDa
Observed MW17kDa, 35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat
Cellular locationCytoplasm
Customer validation

WB(HeLa, Human uterine stromal cells, B6, NMuMG, P. notoginseng, Human, Mouse, Rat, Pig, chicken)

IHC(Mouse kidney, Rat brain)

ABclonal:Western blot - CASP3 Polyclonal Antibody (A2156) }

Western blot - CASP3 Polyclonal Antibody (A2156)

Western blot analysis of extracts of Jurkat cells, using CASP3 antibody (A2156) at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - CASP3 Polyclonal Antibody (A2156) }

Western blot - CASP3 Polyclonal Antibody (A2156)

Western blot analysis of extracts from normal (control) and CASP3 knockout (KO) 293T cells, using CASP3 antibody (A2156) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - CASP3 Polyclonal Antibody (A2156) }

Immunohistochemistry - CASP3 Polyclonal Antibody (A2156)

Immunohistochemistry of paraffin-embedded rat brain using CASP3 antibody (A2156) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CASP3 Polyclonal Antibody (A2156) }

Immunohistochemistry - CASP3 Polyclonal Antibody (A2156)

Immunohistochemistry of paraffin-embedded rat spleen using CASP3 antibody (A2156) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CASP3 Polyclonal Antibody (A2156) }

Immunohistochemistry - CASP3 Polyclonal Antibody (A2156)

Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody (A2156) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CASP3 Polyclonal Antibody (A2156) }

Immunohistochemistry - CASP3 Polyclonal Antibody (A2156)

Immunohistochemistry of paraffin-embedded human placenta using CASP3 antibody (A2156) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CASP3 Polyclonal Antibody (A2156) }

Immunohistochemistry - CASP3 Polyclonal Antibody (A2156)

Immunohistochemistry of paraffin-embedded human gastric cancer using CASP3 antibody (A2156) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CASP3 Polyclonal Antibody (A2156) }

Immunofluorescence - CASP3 Polyclonal Antibody (A2156)

Immunofluorescence analysis of C6 cells using CASP3 antibody (A2156) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CASP3 Polyclonal Antibody (A2156) }

Immunofluorescence - CASP3 Polyclonal Antibody (A2156)

Immunofluorescence analysis of HeLa cells using CASP3 antibody (A2156) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CASP3 Polyclonal Antibody (A2156) }

Immunofluorescence - CASP3 Polyclonal Antibody (A2156)

Immunofluorescence analysis of NIH/3T3 cells using CASP3 antibody (A2156) at dilution of 1:100. Blue: DAPI for nuclear staining.

Submit an review for A2156

  1. Please choose your application, enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Inquire About This Product

Submit your question about A2156 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A2156? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μl sample size available Add to Cart
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order