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Bcl2 Polyclonal Antibody (A2845)

Review (1)Publications (3) Datasheet

ABclonal:Western blot - Bcl2 Polyclonal Antibody (A2845)
ABclonal:Immunofluorescence - Bcl2 Polyclonal Antibody (A2845)
Review
ABclonal: review for Bcl2 Polyclonal Antibody(A2845)

Overview

Product nameBcl2 Polyclonal Antibody
Catalog No.A2845
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 81-131 of human Bcl2 (NP_000624.2).
SequenceAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFA
Gene ID596
Swiss protP10415
SynonymsBCL2; Bcl-2; PPP1R50; apoptosis regulator Bcl-2
Calculated MW22kDa/26kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:1000
IF 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesRaji, HL-60, U-937
Cellular locationEndoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein
Customer validation

WB(Rat, Mouse)

ABclonal:Western blot - Bcl2 Polyclonal Antibody (A2845) }

Western blot - Bcl2 Polyclonal Antibody (A2845)

Western blot analysis of extracts of various cell lines, using BCL2 antibody (A2845) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Bcl2 Polyclonal Antibody (A2845) }

Immunofluorescence - Bcl2 Polyclonal Antibody (A2845)

Immunofluorescence analysis of L929 cells using BCL2 antibody (A2845) at dilution of 1:100. Blue: DAPI for nuclear staining.

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