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BTC Polyclonal Antibody (A2588)

Datasheet

ABclonal:Western blot - BTC Polyclonal Antibody (A2588)

Overview

Product nameBTC Polyclonal Antibody
Catalog No.A2588
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 32-178 of human BTC (NP_001720.1).
SequenceDGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVCDEGYIGARCERVDLFYLRGDRGQILVICLIAVMVVFIILVIGVCTCCHPLRKRRKRKKKEEEMETLGKDITPINEDIEETNIA
Gene ID685
Swiss protP35070
SynonymsBTC; betacellulin
Calculated MW19kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesOVCAR3, PC-3
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein, extracellular space
ABclonal:Western blot - BTC Polyclonal Antibody (A2588) }

Western blot - BTC Polyclonal Antibody (A2588)

Western blot analysis of extracts of various cell lines, using BTC antibody (A2588) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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