Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

BMPR2 Rabbit pAb (A5666)

Publications (3) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - BMPR2 Rabbit pAb (A5666)

Western blot analysis of various lysates, using BMPR2 antibody (A5666) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunofluorescence - BMPR2 Rabbit pAb (A5666)

Immunofluorescence analysis of U-2OS cells using BMPR2 antibody (A5666) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - BMPR2 Rabbit pAb (A5666)

Immunofluorescence analysis of HeLa cells using BMPR2 antibody (A5666) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Overview

Product name BMPR2 Rabbit pAb
Catalog No. A5666
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 27-150 of human BMPR2 (NP_001195.2).
Sequence SQNQERLCAFKDPYQQDLGIGESRISHENGTILCSKGSTCYGLWEKSKGDINLVKQGCWSHIGDPQECHYEECVVTTTPPSIQNGTYRFCCCSTDLCNVNFTENFPPPDTTPLSPPHSFNRDET
Gene ID 659
Swiss prot Q13873
Synonyms BMR2; PPH1; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II; BMPR2
Calculated MW 115kDa
Observed MW 100kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, NIH/3T3, PC-12
Cellular location Cell membrane, Single-pass type I membrane protein
Customer validation

WB (Rattus norvegicus, Homo sapiens)

IF (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

BMPR2 Rabbit pAb images

ABclonal:Western blot - BMPR2 Rabbit pAb (A5666)}

Western blot - BMPR2 Rabbit pAb (A5666)

Western blot analysis of various lysates, using BMPR2 antibody (A5666) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunofluorescence - BMPR2 Rabbit pAb (A5666)}

Immunofluorescence - BMPR2 Rabbit pAb (A5666)

Immunofluorescence analysis of U-2OS cells using BMPR2 antibody (A5666) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - BMPR2 Rabbit pAb (A5666)}

Immunofluorescence - BMPR2 Rabbit pAb (A5666)

Immunofluorescence analysis of HeLa cells using BMPR2 antibody (A5666) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on BMPR2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BMPR2. (Distance between topics and target gene indicate popularity.) BMPR2

* Data provided by citexs.com, for reference only.

Publishing research using A5666? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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