Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

BCAT2 Rabbit pAb (A7426)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - BCAT2 Rabbit pAb (A7426)

Western blot analysis of various lysates using BCAT2 Rabbit pAb (A7426) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - BCAT2 Rabbit pAb (A7426)

Western blot analysis of various lysates using BCAT2 Rabbit pAb (A7426) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - BCAT2 Rabbit pAb (A7426)

Immunohistochemistry analysis of BCAT2 in paraffin-embedded rat brain using BCAT2 Rabbit pAb (A7426) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BCAT2 Rabbit pAb (A7426)

Immunohistochemistry analysis of BCAT2 in paraffin-embedded mouse kidney using BCAT2 Rabbit pAb (A7426) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - BCAT2 Rabbit pAb (A7426)

Immunofluorescence analysis of NIH-3T3 cells using BCAT2 Rabbit pAb (A7426) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name BCAT2 Rabbit pAb
Catalog No. A7426
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 28-392 of human BCAT2 (NP_001181.2).
Sequence ASSSFKAADLQLEMTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV
Gene ID 587
Swiss prot O15382
Synonyms BCAM; BCT2; HVLI; PP18; BCATM; BCAT2
Calculated MW 44kDa
Observed MW 39kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples MCF7, Jurkat, Mouse brain, Rat kidney
Cellular location Cytoplasm, Mitochondrion
Customer validation

FC (Mus musculus)

IF (Mus musculus)

WB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

BCAT2 Rabbit pAb images

ABclonal:Western blot - BCAT2 Rabbit pAb (A7426)}

Western blot - BCAT2 Rabbit pAb (A7426)

Western blot analysis of various lysates using BCAT2 Rabbit pAb (A7426) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - BCAT2 Rabbit pAb (A7426)}

Western blot - BCAT2 Rabbit pAb (A7426)

Western blot analysis of various lysates using BCAT2 Rabbit pAb (A7426) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - BCAT2 Rabbit pAb (A7426)}

Immunohistochemistry - BCAT2 Rabbit pAb (A7426)

Immunohistochemistry analysis of BCAT2 in paraffin-embedded rat brain using BCAT2 Rabbit pAb (A7426) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - BCAT2 Rabbit pAb (A7426)}

Immunohistochemistry - BCAT2 Rabbit pAb (A7426)

Immunohistochemistry analysis of BCAT2 in paraffin-embedded mouse kidney using BCAT2 Rabbit pAb (A7426) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - BCAT2 Rabbit pAb (A7426)}

Immunofluorescence - BCAT2 Rabbit pAb (A7426)

Immunofluorescence analysis of NIH-3T3 cells using BCAT2 Rabbit pAb (A7426) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on BCAT2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BCAT2. (Distance between topics and target gene indicate popularity.) BCAT2

* Data provided by citexs.com, for reference only.

Publishing research using A7426? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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