Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

WSTF Rabbit pAb (A12545)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - WSTF Rabbit pAb (A12545)

Western blot analysis of various lysates using WSTF Rabbit pAb (A12545) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Overview

Product name WSTF Rabbit pAb
Catalog No. A12545
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human WSTF (NP_115784.1).
Sequence MAPLLGRKPFPLVKPLPGEEPLFTIPHTQEAFRTREEYEARLERYSERIWTCKSTGSSQLTHKEAWEEEQEVAELLKEEFPAWYEKLVLEMVHHNTASLEKLVDTAWLEIMTKYAVGEECDFEVGKEKMLKVKIVKIHPLEKVDEEATEKKSDGACDSPSSDKENSSQIAQDHQKKETVVKEDEGRRESINDRARRSPRKLPTSLKKGERKWAPPKFLPHKYDVKLQNEDKIISNVPADSLIRTERPPNKEIVRYFIRHN
Gene ID 9031
Swiss prot Q9UIG0
Synonyms WSTF; WBSCR9; WBSCR10
Calculated MW 171kDa
Observed MW 185kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples BT-474, SKOV3, HeLa, HepG2, Mouse brain, Rat brain
Cellular location Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

WSTF Rabbit pAb images

ABclonal:Western blot - WSTF Rabbit pAb (A12545)}

Western blot - WSTF Rabbit pAb (A12545)

Western blot analysis of various lysates using WSTF Rabbit pAb (A12545) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Related Targets

Based on existing publications, the following genes are closely related to research on BAZ1B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BAZ1B. (Distance between topics and target gene indicate popularity.) BAZ1B

* Data provided by citexs.com, for reference only.

Publishing research using A12545? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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