Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Bax Rabbit pAb (A15646)

Publications (8) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Bax Rabbit pAb (A15646)

Western blot analysis of various lysates using Bax Rabbit pAb (A15646) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - Bax Rabbit pAb (A15646)

Western blot analysis of various lysates using Bax Rabbit pAb (A15646) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Bax Rabbit pAb (A15646)

Immunohistochemistry analysis of Bax in paraffin-embedded human esophageal cancer using Bax Rabbit pAb (A15646) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Bax Rabbit pAb (A15646)

Immunofluorescence analysis of C6 cells using Bax Rabbit pAb (A15646) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Bax Rabbit pAb (A15646)

Immunofluorescence analysis of NIH/3T3 cells using Bax Rabbit pAb (A15646) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Bax Rabbit pAb (A15646)

Immunofluorescence analysis of L929 cells using Bax Rabbit pAb (A15646) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Bax Rabbit pAb (A15646)

Immunoprecipitation analysis of 200ug extracts of Raji cells using 3ug Bax antibody (A15646 1:100). Western blot was performed from the immunoprecipitate using Bax antibody (A15646) at a dilition of 1:1000.

You may also interested in:

Overview

Product name Bax Rabbit pAb
Catalog No. A15646
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

BAX (also known as BCL2 Associated X, Bcl-2-Like Protein 4, Bcl2-L-4, BCL2L4) is a member of the BCL2 family of proteins that play a key role in the regulation of apoptosis in higher eukaryotes (https://www.uniprot.org/uniprot/Q07812). BAX comprises 4 Bcl-2 homology domains (BH1-BH4) and a C-terminal transmembrane domain. In healthy mammalian cells, BAX is localized to the cytoplasm through its interaction with the anti-apoptotic BL-2 family members BCL2L1/Bcl-xL . In response to apoptotic stimuli, however, BAX undergoes a conformational change that causes it to translocate to the outer mitochondrial membrane where it initiates the mitochondrial pathway of apoptosis via two potential mechanisms. Firstly, upon translocation to the outer mitochondrial membrane, BAX interacts with the mitochondrial voltage-dependent anion channel (VDAC) leading to the opening of the channel, loss of membrane potential, and the release of cytochrome c from the mitochondrion . The release of cytochrome C into the cytoplasm leads to the activation of Caspase3, initiating apoptosis. Secondly, activated BAX forms homodimers, which then assemble into oligomers on the mitochondrial outer membrane to create pores that permeabilize the mitochondrion leading to the release of cytochrome C.BAX has been shown to be involved in p53-mediated apoptosis. Expression of the human bax gene has been shown to be directly regulated by p53, and the bax promoter contains four motifs with homology to consensus p53-binding sites. Furthermore, p53 directly interacts with BAX to promote its activation.

Immunogen information

Immunogen Recombinant Protein corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).
Sequence MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF
Gene ID 581
Swiss prot Q07812
Synonyms BCL2 Associated X; Bcl-2-Like Protein 4; Bcl2-L-4; BCL2L4; BAX
Calculated MW 21kDa
Observed MW 21kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples 293T, HT-1080, Raji, HeLa, Mouse lung, Mouse testis, Rat testis, Rat ovary
Cellular location Cytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein
Customer validation

WB (Mus musculus, Rattus norvegicus, Homo sapiens)

IF (Homo sapiens)

IP (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Bax Rabbit pAb images

ABclonal:Western blot - Bax Rabbit pAb (A15646)}

Western blot - Bax Rabbit pAb (A15646)

Western blot analysis of various lysates using Bax Rabbit pAb (A15646) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - Bax Rabbit pAb (A15646)}

Western blot - Bax Rabbit pAb (A15646)

Western blot analysis of various lysates using Bax Rabbit pAb (A15646) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Bax Rabbit pAb (A15646)}

Immunohistochemistry - Bax Rabbit pAb (A15646)

Immunohistochemistry analysis of Bax in paraffin-embedded human esophageal cancer using Bax Rabbit pAb (A15646) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Bax Rabbit pAb (A15646)}

Immunofluorescence - Bax Rabbit pAb (A15646)

Immunofluorescence analysis of C6 cells using Bax Rabbit pAb (A15646) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bax Rabbit pAb (A15646)}

Immunofluorescence - Bax Rabbit pAb (A15646)

Immunofluorescence analysis of NIH/3T3 cells using Bax Rabbit pAb (A15646) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bax Rabbit pAb (A15646)}

Immunofluorescence - Bax Rabbit pAb (A15646)

Immunofluorescence analysis of L929 cells using Bax Rabbit pAb (A15646) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Bax Rabbit pAb (A15646)}

Immunoprecipitation - Bax Rabbit pAb (A15646)

Immunoprecipitation analysis of 200ug extracts of Raji cells using 3ug Bax antibody (A15646 1:100). Western blot was performed from the immunoprecipitate using Bax antibody (A15646) at a dilition of 1:1000.

Inquire About This Product

Submit your question about A15646 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on BAX. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BAX. (Distance between topics and target gene indicate popularity.) BAX

* Data provided by citexs.com, for reference only.

Publishing research using A15646? Please let us know so that we can cite the reference in this datasheet.

Antibodies (10)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order