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BAX Polyclonal Antibody (A0207)

Reviews (3)Publications (5) Datasheet

ABclonal:Western blot - BAX antibody (A0207)
ABclonal:Immunohistochemistry - Bax antibody (A0207)
ABclonal:Immunofluorescence - BAX antibody (A0207)

Overview

Product nameBAX Polyclonal Antibody
Catalog No.A0207
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human BAX (NP_620116.1).
SequenceMDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF
Gene ID581
Swiss protQ07812
SynonymsBCL2L4
Calculated MW4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
Observed MW21kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRaji
Cellular locationCytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein
Customer validation

WB(Human skin T cell lymphoma, Hut102, HCT116, Rat)

ABclonal:Western blot - BAX antibody (A0207)}

Western blot - BAX antibody (A0207)

Western blot analysis of extracts of Raji cells, using BAX antibody (A0207) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Bax antibody (A0207)}

Immunohistochemistry - Bax antibody (A0207)

Immunohistochemistry of paraffin-embedded mouse lung using Bax antibody (A0207) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - BAX antibody (A0207)}

Immunofluorescence - BAX antibody (A0207)

Immunofluorescence analysis of NIH/3T3 cells using BAX antibody (A0207) at dilution of 1:100. Blue: DAPI for nuclear staining.

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