Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

BAG5 Rabbit pAb (A5794)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - BAG5 Rabbit pAb (A5794)

Western blot analysis of extracts of various cell lines, using BAG5 antibody (A5794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - BAG5 Rabbit pAb (A5794)

Immunohistochemistry analysis of paraffin-embedded human stomach using BAG5 antibody (A5794) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BAG5 Rabbit pAb (A5794)

Immunohistochemistry analysis of paraffin-embedded rat brain using BAG5 antibody (A5794) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Overview

Product name BAG5 Rabbit pAb
Catalog No. A5794
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 198-447 of human BAG5 (NP_004864.1).
Sequence GVLIALLMGVNNNETCRHLSCVLSGLIADLDALDVCGRTEIRNYRREVVEDINKLLKYLDLEEEADTTKAFDLRQNHSILKIEKVLKRMREIKNELLQAQNPSELYLSSKTELQGLIGQLDEVSLEKNPCIREARRRAVIEVQTLITYIDLKEALEKRKLFACEEHPSHKAVWNVLGNLSEIQGEVLSFDGNRTDKNYIRLEELLTKQLLALDAVDPQGEEKCKAARKQAVRLAQNILSYLDLKSDEWEY
Gene ID 9529
Swiss prot Q9UL15
Synonyms BAG-5; CMD2F; BAG5
Calculated MW 51kDa
Observed MW 51kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples SH-SY5Y, MCF7, Mouse lung, Mouse testis, Rat brain, Rat lung
Cellular location cytosol, mitochondrion, nucleus, perinuclear region of cytoplasm

Documents

Certificate of Compliance

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Lot number

Research Area

BAG5 Rabbit pAb images

ABclonal:Western blot - BAG5 Rabbit pAb (A5794)}

Western blot - BAG5 Rabbit pAb (A5794)

Western blot analysis of extracts of various cell lines, using BAG5 antibody (A5794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - BAG5 Rabbit pAb (A5794)}

Immunohistochemistry - BAG5 Rabbit pAb (A5794)

Immunohistochemistry analysis of paraffin-embedded human stomach using BAG5 antibody (A5794) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - BAG5 Rabbit pAb (A5794)}

Immunohistochemistry - BAG5 Rabbit pAb (A5794)

Immunohistochemistry analysis of paraffin-embedded rat brain using BAG5 antibody (A5794) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on BAG5. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BAG5. (Distance between topics and target gene indicate popularity.) BAG5

* Data provided by citexs.com, for reference only.

Publishing research using A5794? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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