Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

BAG1 Rabbit pAb (A1104)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - BAG1 Rabbit pAb (A1104)

Western blot analysis of extracts of various cell lines, using BAG1 antibody (A1104) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - BAG1 Rabbit pAb (A1104)

Immunofluorescence analysis of U2OS cells using BAG1 antibody (A1104). Blue: DAPI for nuclear staining.

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Overview

Product name BAG1 Rabbit pAb
Catalog No. A1104
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human BAG1 (NP_001165886.1).
Sequence MNRSQEVTRDEESTRSEEVTREEMAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEMETPLSALGIQDGCRVMLIGKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE
Gene ID 573
Swiss prot Q99933
Synonyms HAP; BAG-1; RAP46; BAG1
Calculated MW 39kDa
Observed MW 43kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples SW620, Mouse kidney
Cellular location Cytoplasm, Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Research Area

BAG1 Rabbit pAb images

ABclonal:Western blot - BAG1 Rabbit pAb (A1104)}

Western blot - BAG1 Rabbit pAb (A1104)

Western blot analysis of extracts of various cell lines, using BAG1 antibody (A1104) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - BAG1 Rabbit pAb (A1104)}

Immunofluorescence - BAG1 Rabbit pAb (A1104)

Immunofluorescence analysis of U2OS cells using BAG1 antibody (A1104). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on BAG1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BAG1. (Distance between topics and target gene indicate popularity.) BAG1

* Data provided by citexs.com, for reference only.

Publishing research using A1104? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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