Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Western blot analysis of various lysates using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at 1:500 dilution.HeLa cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunohistochemistry analysis of Acetyl-Histone H4-K12 in paraffin-embedded human appendix using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunohistochemistry analysis of Acetyl-Histone H4-K12 in paraffin-embedded rat ovary using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunohistochemistry analysis of Acetyl-Histone H4-K12 in paraffin-embedded mouse testis using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunohistochemistry analysis of Acetyl-Histone H4-K12 in paraffin-embedded mouse spinal cord using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Overview

Product name Acetyl-Histone H4-K12 Rabbit pAb
Catalog No. A14227
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Immunogen information

Immunogen A synthetic acetylated peptide around K12 of human Histone H4 (NP_001029249.1).
Sequence GLGKG
Gene ID 8359
Swiss prot P62805
Synonyms H4/I; H4C1; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FI; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; HIST1H4B; Acetyl-Histone H4-K12
Calculated MW 11kDa
Observed MW 11kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples C6, HeLa
Cellular location Chromosome, Nucleus
Customer validation

WB (Rattus norvegicus, Saccharomyces cerevisiae)

Documents

Certificate of Compliance

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Lot number

Research Area

Acetyl-Histone H4-K12 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H4-K12 Rabbit pAb (A14227)}

Western blot - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Western blot analysis of various lysates using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at 1:500 dilution.HeLa cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)}

Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunohistochemistry analysis of Acetyl-Histone H4-K12 in paraffin-embedded human appendix using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)}

Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunohistochemistry analysis of Acetyl-Histone H4-K12 in paraffin-embedded rat ovary using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)}

Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunohistochemistry analysis of Acetyl-Histone H4-K12 in paraffin-embedded mouse testis using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)}

Immunohistochemistry - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunohistochemistry analysis of Acetyl-Histone H4-K12 in paraffin-embedded mouse spinal cord using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Acetyl-Histone H4-K12 Rabbit pAb (A14227)}

Immunofluorescence - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H4-K12 Rabbit pAb (A14227)}

Immunofluorescence - Acetyl-Histone H4-K12 Rabbit pAb (A14227)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 Rabbit pAb (A14227) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on H4C2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H4C2. (Distance between topics and target gene indicate popularity.) H4C2

* Data provided by citexs.com, for reference only.

Publishing research using A14227? Please let us know so that we can cite the reference in this datasheet.

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