Products > Catalog Antibodies > Acetylated Antibodies

Acetyl-Histone H3-K18 pAb (A7257)

Datasheet

ABclonal:Western blot - Acetyl-Histone H3-K18 pAb (A7257)
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)

Overview

Product nameAcetyl-Histone H3-K18 pAb
Catalog No.A7257
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic acetylated peptide corresponding to residues surrounding K18 of human H3
SequenceEmail for sequence
Gene ID8356
Swiss protP68431
SynonymsH3/j; H3FJ
Calculated MW15kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:1000 - 1:3000
IHC 1:200 - 1:500
IF 1:500 - 1:1000
IP 1:200 - 1:500
CHIP 1:50 - 1:100
CHIPseq 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesBT-474, 293T, H3
Cellular locationChromosome, Nucleus
ABclonal:Western blot - Acetyl-Histone H3-K18 pAb (A7257) }

Western blot - Acetyl-Histone H3-K18 pAb (A7257)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K18 antibody (A7257) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257) }

Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)

Immunohistochemistry of paraffin-embedded rat ovary using H3K18ac antibody (A7257) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257) }

Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)

Immunohistochemistry of paraffin-embedded rat brain using H3K18ac antibody (A7257) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257) }

Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)

Immunohistochemistry of paraffin-embedded human stomach using H3K18ac antibody (A7257) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257) }

Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)

Immunohistochemistry of paraffin-embedded human gastric cancer using H3K18ac antibody (A7257) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257) }

Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)

Immunohistochemistry of paraffin-embedded mouse liver using H3K18ac antibody (A7257) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257) }

Immunohistochemistry - Acetyl-Histone H3-K18 pAb (A7257)

Immunohistochemistry of paraffin-embedded mouse brain using H3K18ac antibody (A7257) at dilution of 1:100 (40x lens).

Submit an review for A7257

  1. Please choose your application, enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Inquire About This Product

Submit your question about A7257 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Contact us to order