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ATF4 Polyclonal Antibody (A0201)

Publication (2) Datasheet

ABclonal:Western blot - ATF4 Polyclonal Antibody (A0201)
ABclonal:Immunofluorescence - ATF4 Polyclonal Antibody (A0201)

Overview

Product nameATF4 Polyclonal Antibody
Catalog No.A0201
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4 (NP_001666.2).
SequenceMTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP
Gene ID468
Swiss protP18848
SynonymsATF4; CREB-2; CREB2; TAXREB67; TXREB; activating transcription factor 4
Calculated MW38kDa
Observed MW50kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa
Cellular locationCell membrane, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Customer validation

WB(Mouse, Rat)

ABclonal:Western blot - ATF4 Polyclonal Antibody (A0201) }

Western blot - ATF4 Polyclonal Antibody (A0201)

Western blot analysis of extracts of HeLa cells, using ATF4 antibody (A0201) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunofluorescence - ATF4 Polyclonal Antibody (A0201) }

Immunofluorescence - ATF4 Polyclonal Antibody (A0201)

Immunofluorescence analysis of HeLa cells using ATF4 antibody (A0201) at dilution of 1:100. Blue: DAPI for nuclear staining.

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