Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

APC Rabbit pAb (A2818)

Reviews (2) Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - APC Rabbit pAb (A2818)

Western blot analysis of extracts of various cell lines, using APC antibody (A2818) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - APC Rabbit pAb (A2818)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using APC Rabbit pAb (A2818) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - APC Rabbit pAb (A2818)

Immunofluorescence analysis of PC-12 cells using APC antibody (A2818) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for APC Rabbit pAb(A2818)
Review
ABclonal: review for APC Rabbit pAb(A2818)

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Overview

Product name APC Rabbit pAb
Catalog No. A2818
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Mutations in the APC gene have been found to occur in most colorectal cancers, where disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 2600-2760 of human APC (NP_000029.2).
Sequence QSKENQVSAKGTWRKIKENEFSPTNSTSQTVSSGATNGAESKTLIYQMAPAVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKANPNIKDSKDNQAKQNVGNGSVPMRTVGLENRLNSFIQVDAPDQKGTEIKPGQNNPVPVSETNESSIVERT
Gene ID 324
Swiss prot P25054
Synonyms GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46; APC
Calculated MW 312kDa
Observed MW 150kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples SW480, Mouse brain, Rat brain
Cellular location Cell junction, Cell membrane, Cell projection, Cytoplasm, adherens junction, cytoskeleton, lamellipodium, ruffle membrane
Customer validation

WB (Human chronic myeloid leukemia cells)

IF (HeLa cellsHomo sapiens)

ICC (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

APC Rabbit pAb images

ABclonal:Western blot - APC Rabbit pAb (A2818)}

Western blot - APC Rabbit pAb (A2818)

Western blot analysis of extracts of various cell lines, using APC antibody (A2818) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - APC Rabbit pAb (A2818)}

Immunohistochemistry - APC Rabbit pAb (A2818)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using APC Rabbit pAb (A2818) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - APC Rabbit pAb (A2818)}

Immunofluorescence - APC Rabbit pAb (A2818)

Immunofluorescence analysis of PC-12 cells using APC antibody (A2818) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on APC. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to APC. (Distance between topics and target gene indicate popularity.) APC

* Data provided by citexs.com, for reference only.

Publishing research using A2818? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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