Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CD13/ANPEP Rabbit pAb (A5662)

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CD13/ANPEP Rabbit pAb (A5662)

Western blot analysis of extracts of various cell lines, using CD13/ANPEP antibody (A5662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - CD13/ANPEP Rabbit pAb (A5662)

Western blot analysis of extracts of OVCAR3 cells, using CD13/ANPEP antibody (A5662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CD13/ANPEP Rabbit pAb (A5662)

Immunohistochemistry analysis of paraffin-embedded human placenta using CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD13/ANPEP Rabbit pAb (A5662)

Immunohistochemistry analysis of paraffin-embedded mouse intestin using CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD13/ANPEP Rabbit pAb (A5662)

Immunohistochemistry analysis of paraffin-embedded rat kidney using CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of NIH/3T3 cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of HeLa cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of C6 cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Overview

Product name CD13/ANPEP Rabbit pAb
Catalog No. A5662
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. This membrane-bound zinc metalloprotease is known to serve as a receptor for the HCoV-229E alphacoronavirus as well as other non-human coronaviruses. This gene has also been shown to promote angiogenesis, tumor growth, and metastasis and defects in this gene are associated with various types of leukemia and lymphoma.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 668-967 of human CD13/CD13/ANPEP (NP_001141.2).
Sequence LASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK
Gene ID 290
Swiss prot P15144
Synonyms APN; AP-M; AP-N; CD13; LAP1; P150; PEPN; hAPN; GP150; CD13/ANPEP
Calculated MW 110kDa
Observed MW 150kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples OVCAR3, Mouse kidney, Rat kidney
Cellular location Cell membrane, Cytoplasm, Single-pass type II membrane protein, cytosol
Customer validation

WB (Sus scrofa, Homo sapiens, Mus musculus)

ELISA (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CD13/ANPEP Rabbit pAb images

ABclonal:Western blot - CD13/ANPEP Rabbit pAb (A5662)}

Western blot - CD13/ANPEP Rabbit pAb (A5662)

Western blot analysis of extracts of various cell lines, using CD13/ANPEP antibody (A5662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - CD13/ANPEP Rabbit pAb (A5662)}

Western blot - CD13/ANPEP Rabbit pAb (A5662)

Western blot analysis of extracts of OVCAR3 cells, using CD13/ANPEP antibody (A5662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CD13/ANPEP Rabbit pAb (A5662)}

Immunohistochemistry - CD13/ANPEP Rabbit pAb (A5662)

Immunohistochemistry analysis of paraffin-embedded human placenta using CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD13/ANPEP Rabbit pAb (A5662)}

Immunohistochemistry - CD13/ANPEP Rabbit pAb (A5662)

Immunohistochemistry analysis of paraffin-embedded mouse intestin using CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD13/ANPEP Rabbit pAb (A5662)}

Immunohistochemistry - CD13/ANPEP Rabbit pAb (A5662)

Immunohistochemistry analysis of paraffin-embedded rat kidney using CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)}

Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of NIH/3T3 cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)}

Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of HeLa cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)}

Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of C6 cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on ANPEP. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ANPEP. (Distance between topics and target gene indicate popularity.) ANPEP

* Data provided by citexs.com, for reference only.

Publishing research using A5662? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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