Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

AMPKa1/AMPKa2 Rabbit pAb (A12718)

Publications (11) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Western blot analysis of various lysates, using AMPKa1/AMPKa2 Rabbit pAb (A12718) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded human colon using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded mouse kidney using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded rat lung using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Immunofluorescence analysis of MCF7 cells using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Immunofluorescence analysis of NIH/3T3 cells using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name AMPKa1/AMPKa2 Rabbit pAb
Catalog No. A12718
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 13-300 of human PRKAA1/PRKAA2 (NP_006242.5).
Sequence AEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMID
Gene ID 55625563
Swiss prot Q13131P54646
Synonyms AMPKa1/AMPKa2
Calculated MW 64kDa/65kDa/62kDa
Observed MW 62kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples MCF7, 293F, HeLa, C6
Cellular location apical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus
Customer validation

WB (Homo sapiens, Mus musculus, Rattus norvegicus)

IHC (Rattus norvegicus)

IF (Rattus norvegicus)

ELISA (Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

AMPKa1/AMPKa2 Rabbit pAb images

ABclonal:Western blot - AMPKa1/AMPKa2 Rabbit pAb (A12718)}

Western blot - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Western blot analysis of various lysates, using AMPKa1/AMPKa2 Rabbit pAb (A12718) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunohistochemistry - AMPKa1/AMPKa2 Rabbit pAb (A12718)}

Immunohistochemistry - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded human colon using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - AMPKa1/AMPKa2 Rabbit pAb (A12718)}

Immunohistochemistry - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded mouse kidney using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - AMPKa1/AMPKa2 Rabbit pAb (A12718)}

Immunohistochemistry - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded rat lung using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - AMPKa1/AMPKa2 Rabbit pAb (A12718)}

Immunofluorescence - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Immunofluorescence analysis of MCF7 cells using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - AMPKa1/AMPKa2 Rabbit pAb (A12718)}

Immunofluorescence - AMPKa1/AMPKa2 Rabbit pAb (A12718)

Immunofluorescence analysis of NIH/3T3 cells using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PRKAA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PRKAA1. (Distance between topics and target gene indicate popularity.) PRKAA1

* Data provided by citexs.com, for reference only.

Publishing research using A12718? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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