Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

AFF4 Rabbit pAb (A4644)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - AFF4 Rabbit pAb (A4644)

Western blot analysis of extracts of various cell lines, using AFF4 antibody (A4644) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 30s.

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Overview

Product name AFF4 Rabbit pAb
Catalog No. A4644
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23).

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human AFF4 (NP_055238.1).
Sequence MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANW
Gene ID 27125
Swiss prot Q9UHB7
Synonyms MCEF; CHOPS; AF5Q31; AFF4
Calculated MW 127kDa
Observed MW 150kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples U-87MG, HeLa, 293T, LO2, MCF7, Mouse pancreas
Cellular location Nucleus
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

AFF4 Rabbit pAb images

ABclonal:Western blot - AFF4 Rabbit pAb (A4644)}

Western blot - AFF4 Rabbit pAb (A4644)

Western blot analysis of extracts of various cell lines, using AFF4 antibody (A4644) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on AFF4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to AFF4. (Distance between topics and target gene indicate popularity.) AFF4

* Data provided by citexs.com, for reference only.

Publishing research using A4644? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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