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ACVR1B Polyclonal Antibody (A5453)

Publication (1) Datasheet

ABclonal:Western blot - ACVR1B Polyclonal Antibody (A5453)


Product nameACVR1B Polyclonal Antibody
Catalog No.A5453
Host speciesRabbit
Purification methodAffinity purification
This gene encodes an activin A type IB receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. This protein is a type I receptor which is essential for signaling. Mutations in this gene are associated with pituitary tumors. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-126 of human ACVR1B (NP_004293.1).
Gene ID91
Swiss protP36896
Calculated MW51kDa/53kDa/54kDa/56kDa/61kDa
Observed MW49kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesSW480, Mouse kidney
Cellular locationCell membrane, Single-pass type I membrane protein
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ABclonal:Western blot - ACVR1B Polyclonal Antibody (A5453) }

Western blot - ACVR1B Polyclonal Antibody (A5453)

Western blot analysis of extracts of various cell lines, using ACVR1B antibody (A5453) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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